Refining hemodynamic correction in in vivo wide-field fluorescent imaging through linear regression analysis

Jing Li; Fan Yang; Kathleen Zhang; Shiqiang Wu; James Niemeyer; Mingrui Zhao; Peijuan Luo; Nan Li; Rongxin Li; Dan Li; Weihong Lin; Jyun-You Liou; Theodore H Schwartz; Hongtao Ma

doi:10.1016/j.neuroimage.2024.120816

Refining hemodynamic correction in in vivo wide-field fluorescent imaging through linear regression analysis

Neuroimage. 2024 Oct 1:299:120816. doi: 10.1016/j.neuroimage.2024.120816. Epub 2024 Aug 30.

Authors

Jing Li¹, Fan Yang¹, Kathleen Zhang², Shiqiang Wu³, James Niemeyer⁴, Mingrui Zhao⁴, Peijuan Luo¹, Nan Li⁵, Rongxin Li⁵, Dan Li⁶, Weihong Lin⁷, Jyun-You Liou⁸, Theodore H Schwartz⁹, Hongtao Ma¹⁰

Affiliations

¹ Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China; Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA.
² Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA; Stuyvesant High School, New York, New York 10282, USA.
³ Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA; Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
⁴ Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA.
⁵ Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
⁶ Department of Radiology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
⁷ Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin 130021, China. Electronic address: [email protected].
⁸ Department of Anesthesiology, Weill Cornell Medicine, New York, New York, NY 10065, USA.
⁹ Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA. Electronic address: [email protected].
¹⁰ Department of Neurological Surgery, New York Presbyterian Hospital, Weill Cornell Medicine of Cornell University, 525 East 68th Street, Box 99, New York, NY 10065, USA. Electronic address: [email protected].

PMID: 39209071
DOI: 10.1016/j.neuroimage.2024.120816

Abstract

Accurate interpretation of in vivo wide-field fluorescent imaging (WFFI) data requires precise separation of raw fluorescence signals into neural and hemodynamic components. The classical Beer-Lambert law-based approach, which uses concurrent 530-nm illumination to estimate relative changes in cerebral blood volume (CBV), fails to account for the scattering and reflection of 530-nm photons from non-neuronal components leading to biased estimates of CBV changes and subsequent misrepresentation of neural activity. This study introduces a novel linear regression approach designed to overcome this limitation. This correction provides a more reliable representation of CBV changes and neural activity in fluorescence data. Our method is validated across multiple datasets, demonstrating its superiority over the classical approach.

Keywords: Beer-lambert law; Functional hemodynamic changes; Linear regression; Noise subtraction method; Wide-field fluorescent imaging.

MeSH terms

Animals
Brain / blood supply
Brain / diagnostic imaging
Cerebral Blood Volume / physiology
Cerebrovascular Circulation / physiology
Hemodynamics* / physiology
Humans
Image Processing, Computer-Assisted / methods
Linear Models
Male
Optical Imaging / methods