Integration of mass cytometry and mass spectrometry imaging for spatially resolved single-cell metabolic profiling

Nat Methods. 2024 Oct;21(10):1796-1800. doi: 10.1038/s41592-024-02392-6. Epub 2024 Aug 29.

Abstract

The integration of spatial omics technologies can provide important insights into the biology of tissues. Here we combined mass spectrometry imaging-based metabolomics and imaging mass cytometry-based immunophenotyping on a single tissue section to reveal metabolic heterogeneity at single-cell resolution within tissues and its association with specific cell populations such as cancer cells or immune cells. This approach has the potential to greatly increase our understanding of tissue-level interplay between metabolic processes and their cellular components.

MeSH terms

  • Animals
  • Flow Cytometry / methods
  • Humans
  • Immunophenotyping / methods
  • Mass Spectrometry / methods
  • Metabolome
  • Metabolomics* / methods
  • Mice
  • Single-Cell Analysis* / methods