Antimicrobial susceptibility of commensal Neisseria spp. in parents and their children in Belgium: a cross-sectional survey

FEMS Microbiol Lett. 2024 Jan 9:371:fnae069. doi: 10.1093/femsle/fnae069.

Abstract

Background: commensal Neisseria species are part of the oropharyngeal microbiome and play an important role in nitrate reduction and protecting against colonization by pathogenic bacteria. They do, however, also serve as a reservoir of antimicrobial resistance. Little is known about the prevalence of these species in the general population, how this varies by age and how antimicrobial susceptibility varies between species.

Methods: we assessed the prevalence and antimicrobial susceptibility of commensal Neisseria species in the parents (n = 38) and children (n = 50) of 35 families in Belgium.

Results: various commensal Neisseria (n = 5) could be isolated from the participants. Most abundant were N. subflava and N. mucosa. Neisseria subflava was detected in 77 of 88 (87.5%) individuals and N. mucosa in 64 of 88 (72.7%). Neisseria mucosa was more prevalent in children [41/50 (82%)] than parents [23/38 (60.5%); P < .05], while N. bacilliformis was more prevalent in parents [7/36 (19.4%)] than children [2/50 (4%); P < .05]. Neisseria bacilliformis had high ceftriaxone minimum inhibitory concentrations (MICs; median MIC 0.5 mg/l; IQR 0.38-0.75). The ceftriaxone MICs of all Neisseria isolates were higher in the parents than in the children. This could be explained by a higher prevalence of N. bacilliformis in the parents.

Interpretation: the N. bacilliformis isolates had uniformly high ceftriaxone MICs which warrant further investigation.

Keywords: Neisseria; Belgium; commensals; horizontal gene transfer; intrafamilial transmission; oropharynx.

MeSH terms

  • Adolescent
  • Adult
  • Anti-Bacterial Agents* / pharmacology
  • Belgium / epidemiology
  • Child
  • Child, Preschool
  • Cross-Sectional Studies
  • Drug Resistance, Bacterial
  • Female
  • Humans
  • Infant
  • Male
  • Microbial Sensitivity Tests*
  • Middle Aged
  • Neisseria* / drug effects
  • Neisseria* / genetics
  • Neisseria* / isolation & purification
  • Oropharynx / microbiology
  • Parents*
  • Prevalence
  • Young Adult

Substances

  • Anti-Bacterial Agents

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