Impact of cell voltage on synthesis of caproic acid from carbon dioxide and ethanol in direct current powered microbial electrosynthesis cell

Production of medium chain fatty acids (MCFAs) from CO₂ through microbial electrosynthesis (MES) holds great potential. The present study investigated the effect of cathode voltages of - 0.8 V (MES-1), -1.0 V (MES-2) and -1.2 V vs Ag/AgCl (MES-3), on the production of MCFAs from CO₂ and ethanol using an enriched culture. Direct current (DC) power supply was used to maintain constant cathode voltages. The highest amounts of caproic acid were produced in MES-2 at an average concentration of 1.51 ± 0.14 g/L with a maximum selectivity of 68 ± 7 %. Microbial diversity analysis showed abundance of the Clostridiaceae family that allowed chain elongation in all MES reactors. This study shows that potentiostatic control approach for MCFA synthesis, can be replaced by DC power supply in future MES setups. Using selective culture enrichment, MES efficiently produces MCFAs from CO₂ and ethanol, with -1.0 V yielding the highest caproic acid.