Phytolacca americana extract as a quality-enhancing factor for biodegradable double-layered films based on furcellaran and gelatin - Property assessment

Joanna Maria Jasińska; Klaudia Michalska; Michał Szuwarzyński; Tomasz Mazur; Agnieszka Cholewa-Wójcik; Michał Kopeć; Lesław Juszczak; Iwona Kamińska; Nikola Nowak; Ewelina Jamróz

doi:10.1016/j.ijbiomac.2024.135155

Phytolacca americana extract as a quality-enhancing factor for biodegradable double-layered films based on furcellaran and gelatin - Property assessment

Int J Biol Macromol. 2024 Nov;279(Pt 2):135155. doi: 10.1016/j.ijbiomac.2024.135155. Epub 2024 Aug 28.

Affiliations

¹ Department of Chemistry, University of Agriculture, Balicka 122, PL-30-149 Kraków, Poland. Electronic address: [email protected].
² Department of Phytochemistry, Institute of Pharmacology, Polish Academy of Sciences, Kraków PL-31-343, Poland.
³ AGH University of Krakow, Academic Centre for Materials and Nanotechnology, Al. Mickiewicza 30, 30-059 Kraków, Poland.
⁴ Department of Product Packaging, Cracow University of Economics, Rakowicka 27, PL-31-510 Kraków, Poland.
⁵ Department of Agricultural and Environmental Chemistry, University of Agriculture in Kraków, al. Mickiewicza 21, PL-31-120 Kraków, Poland.
⁶ Department of Dietetics and Food Studies, Faculty of Science & Technology, Jan Długosz University in Częstochowa, Armii Krajowej 13/15, PL-42-200 Czestochowa, Poland; Department of Food Analysis and Evaluation of Food Quality, University of Agriculture in Krakow, Balicka 122, PL-30-149 Kraków, Poland.
⁷ Department of Botany, Physiology and Plant Protection, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. Mickiewicza 21, PL-31-120 Kraków, Poland.
⁸ Department of Chemistry, University of Agriculture, Balicka 122, PL-30-149 Kraków, Poland.
⁹ Department of Chemistry, University of Agriculture, Balicka 122, PL-30-149 Kraków, Poland; Department of Product Packaging, Cracow University of Economics, Rakowicka 27, PL-31-510 Kraków, Poland.

PMID: 39214197
DOI: 10.1016/j.ijbiomac.2024.135155

Abstract

For the first time, novel active double-layered films based on furcellaran (FUR) and gelatin (GEL) with the addition of Phytolacca americana (PA) extract were obtained. The 1st layer consisted of FUR and GEL, while the aqueous extract of P. americana berries was added in three different concentrations to the 2nd FUR-based layer. The films were characterised by good mechanical (TS range of 0.0011-0.0013 MPa, EAB range between 30.38 %-33.51 %) and water properties (WVTR range of 574.74-588.49 g/m²xd). Structural analysis (SEM and AFM) confirmed good film structure: regular, without cracks or air bubbles. The films showed antioxidant activity tested via the Folin-Ciocâlteu method (4.77-20.70 mg GAExg^-1), FRAP assay (0.18-3.40 mM TExg^-1) and CUPRAC assay (48.63-53.99 mM TExg^-1). The film with the highest PA concentration (6 %) demonstrated the ability to neutralise free radicals, DPPH^• and ABTS^2+•, at the levels of 1.97 % and 17.34 %, respectively. The ecotoxicity test performed on Lepidium sativum seeds confirmed the lack of ecotoxic film aspects. The biodegradation test indicated that the films are biodegradable. The obtained films can be a good alternative to plastic packaging films (used in the food packaging industry), which are currently a global problem related to the development of post-consumer plastics.

Keywords: Active films; Double-layered films; Furcellaran.

MeSH terms

Alginates
Antioxidants* / chemistry
Antioxidants* / pharmacology
Food Packaging / methods
Gelatin* / chemistry
Phytolacca americana* / chemistry
Plant Extracts* / chemistry
Plant Extracts* / pharmacology
Plant Gums

Substances

Gelatin
Plant Extracts
Antioxidants
furcellaran
Alginates
Plant Gums