Comparison of Three FISH Probes Used By FISH Test For 13q Deletion in Chronic Lymphoid Leukemia

Tania Quintana Hernandez; Asha Grover; Jamin Liu; Kenian Liu

Comparison of Three FISH Probes Used By FISH Test For 13q Deletion in Chronic Lymphoid Leukemia

J Assoc Genet Technol. 2024;50(3):101-102.

Authors

Tania Quintana Hernandez¹, Asha Grover¹, Jamin Liu¹, Kenian Liu²

Affiliations

¹ Cytogenetics Technologist, Moffitt Cancer Center, Tampa, FL.
² Cytogenetics Lab director, Department of Pathology, Moffitt Cancer Center, Tampa, FL.

PMID: 39222518

Abstract

Fluorescence in situ hybridization (FISH) is a reliable method to detect a deletion on chromosome 13q14. Currently, three commercial probes are available for FISH testing in clinical cytogenetics laboratories, RB1, D13S319, and D13S25, with the D13S319 probe most commonly used for 13q deletion. In this study, we compared FISH test results among these three probes in CLL cases with positive 13q deletion. We found that the D13S25 probe set has the highest detection rate of the percentage of the biallelic 13q deletion.