Protocol for volume correlative light X-ray and electron microscopy of endothelial cells in mouse tissue

STAR Protoc. 2024 Sep 20;5(3):103257. doi: 10.1016/j.xpro.2024.103257. Epub 2024 Sep 1.

Abstract

Correlative light and electron microscopy (CLEM) greatly facilitate capturing the ultrastructure of spatially and/or temporally rare events. Here, we present a protocol for targeting regions of interests (ROIs) in tissue endothelial cells (ECs) using X-ray micro-computed tomography (μCT). We describe steps for ROI targeting guided by vasculature patterns and positions of EC nuclei visualized by light and X-ray microscopy. The protocol is applicable to thin or translucent tissues that contain defined landmarks visible in both light and X-ray microscopy. For complete details on the use and execution of this protocol, please refer to Reglero-Real et al.1.

Keywords: Cell Biology; Immunology; Microscopy.

MeSH terms

  • Animals
  • Endothelial Cells* / cytology
  • Endothelial Cells* / ultrastructure
  • Mice
  • Microscopy / methods
  • Microscopy, Electron* / methods
  • X-Ray Microtomography / methods