Spatiotemporal dynamics of microscopic biological barrier visualized by electric-double-layer modulation imaging

Jun Kurosu; Takato Sakamaki; Kaname Kanai; Kana Morishita; Kimio Sumaru; Jun'ya Tsutsumi

doi:10.1016/j.bios.2024.116721

Spatiotemporal dynamics of microscopic biological barrier visualized by electric-double-layer modulation imaging

Biosens Bioelectron. 2024 Dec 15:266:116721. doi: 10.1016/j.bios.2024.116721. Epub 2024 Aug 30.

Authors

Jun Kurosu¹, Takato Sakamaki¹, Kaname Kanai², Kana Morishita³, Kimio Sumaru³, Jun'ya Tsutsumi⁴

Affiliations

¹ Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, 278-8510, Japan.
² Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, 278-8510, Japan.
³ Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan.
⁴ Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, 278-8510, Japan. Electronic address: [email protected].

PMID: 39226753
DOI: 10.1016/j.bios.2024.116721

Abstract

Live-cell label-free imaging of a microscopic biological barrier, generally referred to as 'tight junction', was realized by a recently developed electric-double-layer modulation imaging (EDLMI). The method allowed quantitative imaging of barrier integrity in real time, thus being an upper compatible of transepithelial electrical resistance (TEER) which is a conventional standard technique to evaluate spatially averaged barrier integrity. We demonstrate that the quantitative and real-time imaging capability of EDLMI unveils fundamental dynamics of biological barrier, some of which are totally different from conventional understandings.

Keywords: Absorption enhancer; Intestinal epithelial cell; Label-free; Live-cell imaging; Tight junction; Transepithelial electrical resistance (TEER).

MeSH terms

Biosensing Techniques* / methods
Electric Impedance
Humans
Tight Junctions / metabolism