Rice transcription factor DPF regulates stress-induced biosynthesis of diterpenoid phytoalexins

Biosci Biotechnol Biochem. 2024 Oct 22;88(11):1344-1348. doi: 10.1093/bbb/zbae118.

Abstract

Diterpenoid Phytoalexin Factor (DPF) is a key transcription factor involved in diterpenoid phytoalexin (DP) biosynthesis under non-stressed conditions in rice (Oryza sativa L.). Using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9, DPF knockout rice lines were generated. Treatments with abiotic stresses (copper chloride, ultraviolet light, and jasmonic acid) and biotic stress (blast fungus infection) to the knockout lines revealed that the DPF positively regulates stress-induced DP biosynthesis.

Keywords: jasmonic acid; phytoalexin; rice; stress; transcription factor.

MeSH terms

  • CRISPR-Cas Systems
  • Cyclopentanes / metabolism
  • Diterpenes* / metabolism
  • Gene Expression Regulation, Plant
  • Gene Knockout Techniques
  • Oryza* / genetics
  • Oryza* / metabolism
  • Oryza* / microbiology
  • Oxylipins / metabolism
  • Phytoalexins*
  • Plant Proteins* / genetics
  • Plant Proteins* / metabolism
  • Sesquiterpenes* / metabolism
  • Stress, Physiological*
  • Transcription Factors* / genetics
  • Transcription Factors* / metabolism
  • Ultraviolet Rays

Substances

  • Phytoalexins
  • Sesquiterpenes
  • Transcription Factors
  • Plant Proteins
  • Diterpenes
  • jasmonic acid
  • Oxylipins
  • Cyclopentanes