Background: A common complication of wounds is the excessive production of fibrotic scar tissue, which can lead to hypertrophic scars or keloids. Currently, no treatments with good evidence for preventing excessive scar tissue formation are available. We explored the use of microneedle patches containing small interfering RNA (siRNA) to inhibit SPARC mRNA in reducing the volume of postoperative scars.
Objectives: To compare differences in postoperative scar volume with the daily application of siRNA-embedded dissolving microneedle patches vs. silicone sheets.
Methods: This was an 8-week, single-blinded intraindividually controlled randomized trial at a tertiary dermatological centre. Patients with 2-week-old postoperative wounds were included. Each half of the scar was randomly assigned to the microneedle patch or silicone sheet. Three-dimensional (3D) volumes were obtained from the scars via a high-resolution scanner at days 0, 30 and 60. The trial was registered with the Australian New Zealand Clinical Trials Registry (ACTRN12622000558729).
Results: At day 30, scars treated with microneedle patches had a lower geometric mean volume of 0.79 mm3 vs. scars treated with silicone sheets, with a difference in mean percentage volume reduction of 10.7%. At day 60, scars treated with microneedle patches had a statistically significant lower volume (8.88 mm3) compared with the side treated with silicone sheets (12.77 mm3; P = 0.005), with a difference in mean percentage reduction of 9.7%. Additionally, there was also a statistically significant difference between the percentage reduction in scar volume vs. baseline on the side treated with microneedle patches (mean 83.8%) compared with the side treated with silicone sheets (mean 74.1%).
Conclusions: There was a significantly greater reduction in the volume of postoperative scars on the side of the scar treated with microneedle patches compared with the side treated with silicone sheets. This demonstrates the use of transdermal gene-silencing technology in scar inhibition and that siRNA microneedle patches can be effective and safe in reducing scar tissue formation.
A common complication of wounds to the skin can be the over-production of scar tissue. There are currently no good treatments to prevent excessive scar tissue after surgery. In this study, we used a type of gene technology in special microneedle patches to minimize the formation of excessive scars after surgery. One process in skin that causes excessive scarring is the over-production of a protein called ‘SPARC’. Our microneedle patches contained a piece a tiny synthetic RNA sequence called ‘small interfering RNA’ or ‘siRNA’ for short. This siRNA inhibits the production SPARC and decreases scar tissue formation. We looked at differences in post-operative scars with the daily application of siRNA microneedle patches and silicone sheets that did not contain siRNA (2 weeks after surgery). Each wound was divided in half. The siRNA microneedle patch was applied to one half and a silicone sheet to the other. Scars were measured and compared at 4 and 8 weeks after the application of the patches and sheets. We found that the amount of scar tissue on both sides of the scar was reduced. There was a lower volume of scar tissue on the side with the siRNA microneedle patches than on the side treated with the silicone sheets. No side effects were reported by the people who took part. Our findings suggest that using siRNA microneedle patches for 1 or 2 months after surgery could be used to reduce scar formation. We found that siRNA patches can be effective, safe and easy to use in reducing scar tissue formation in post-operative wounds.
© The Author(s) 2024. Published by Oxford University Press on behalf of British Association of Dermatologists.