Clinical performance of a rapid RT-PCR assay using STANDARD™ M10 SARS-CoV-2 between July 2022 and January 2023 in Korea

Diagn Microbiol Infect Dis. 2024 Dec;110(4):116523. doi: 10.1016/j.diagmicrobio.2024.116523. Epub 2024 Sep 2.

Abstract

Rapid detection of SARS-CoV-2 is essential for clinical management in the emergency department during the COVID-19 pandemic. We evaluated the clinical performance of the recently developed cartridge-based rapid RT-PCR assay (STANDARD M10 SARS-CoV-2) in patients visiting the emergency department from July 2022 to January 2023, which was when the Omicron BA.5 sublineage was predominant in Korea. A total of 534 specimens were subjected to the STANDARD M10 and standard RT-PCR (Allplex SARS-CoV-2) assays. The overall, positive, and negative percent agreements between these two assays were 99.6%, 100%, and 99.6%, respectively. The results showed that compared with the established RT-PCR assay, the STANDARD M10 SARS-CoV-2 assay is a reliable and useful tool for SARS-CoV-2 detection during the study period. The new rapid RT-PCR will expand the diversity in rapid diagnostics and can help resolve the global imbalance associated with the supply of diagnostic resources.

Keywords: COVID-19; Omicron BA.5; Rapid RT-PCR; SARS-CoV-2; STANDARD M10.

MeSH terms

  • Adult
  • Aged
  • COVID-19 Nucleic Acid Testing* / methods
  • COVID-19* / diagnosis
  • COVID-19* / virology
  • Emergency Service, Hospital
  • Female
  • Humans
  • Male
  • Middle Aged
  • Republic of Korea
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Reverse Transcriptase Polymerase Chain Reaction / standards
  • SARS-CoV-2* / genetics
  • SARS-CoV-2* / isolation & purification
  • Sensitivity and Specificity*