Association of genetic variants in CYP3A5, DRD2 and NK1R with opioid overdose

Chem Biol Interact. 2024 Nov 1:403:111242. doi: 10.1016/j.cbi.2024.111242. Epub 2024 Sep 12.

Abstract

In 2023, 3651 Ohioans died because of an opioid overdose. Of those opioid overdoses, 3579 (98%) of which were attributed to fentanyl. We evaluated the association between 180 candidate single nucleotide polymorphisms (SNPs) and self-reported, nonfatal opioid overdose history from a prospective sample of 1301 adult patients (≥18 years of age) seen in three urban emergency departments in Ohio. Candidate SNPs included 120 related to the dopamine reward pathway and 60 related to pharmacokinetics. Of the 821 patients who reported having been exposed to opioids in their lifetime, 95 (11.6%) also reported having experienced an opioid-related overdose. Logistic regression, adjusting for age and biologic sex, was used to characterize the association between each SNP and opioid overdose, correcting for multiple comparisons. Three SNPs, located in three different genes were associated with opioid overdose: increased odds with CYP3A5 (rs776746) and DRD2 (rs4436578), and decreased odds with NKIR (rs6715729). Homozygotic CYP3A5 (rs776746) had the highest adjusted odds ratio (OR) of 6.96 (95% CI [2.45, 29.23]) and homozygotic NK1R (rs6715729) had the lowest OR of 0.28 (95% CI [0.14, 0.54). Given that CYP3A5 (rs776746) has been associated with increased plasma concentrations of fentanyl, rs776746 could potentially be utilized as a prognostic risk indicator for the potential of an opioid overdose. NK1R regulates the expression of the neurokinin-1 receptor, a regulator of respiration and NK1R (rs6715729) represents a novel genetic marker for a decreased risk of opioid overdose risk.

Keywords: Drug overdose; Fentanyl; Pharmacogenetics; SNPs.

MeSH terms

  • Adult
  • Analgesics, Opioid / poisoning
  • Cytochrome P-450 CYP3A* / genetics
  • Cytochrome P-450 CYP3A* / metabolism
  • Female
  • Humans
  • Male
  • Middle Aged
  • Opiate Overdose* / genetics
  • Polymorphism, Single Nucleotide*
  • Receptors, Dopamine D2* / genetics
  • Receptors, Dopamine D2* / metabolism
  • Receptors, Neurokinin-1* / genetics
  • Receptors, Neurokinin-1* / metabolism
  • Young Adult

Substances

  • Cytochrome P-450 CYP3A
  • Receptors, Dopamine D2
  • Receptors, Neurokinin-1
  • DRD2 protein, human
  • CYP3A5 protein, human
  • Analgesics, Opioid