Proposing an Affordable Plasma Device for Polymer Surface Modification and Microbial Inactivation

Molecules. 2024 Sep 9;29(17):4270. doi: 10.3390/molecules29174270.

Abstract

This study proposes an affordable plasma device that utilizes a parallel-plate dielectric barrier discharge geometry with a metallic mesh electrode, featuring a straightforward 3D-printed design. Powered by a high-voltage supply adapted from a cosmetic plasma device, it operates on atmospheric air, eliminating the need for gas flux. Surface modification of polyethylene treated with this device was characterized and showed that the elemental composition after 15 min of plasma treatment decreased the amount of C to ~80 at% due to the insertion of O (~15 at%). Tested against Candida albicans and Staphylococcus aureus, the device achieved a reduction of over 99% in microbial load with exposure times ranging from 1 to 10 min. Simultaneously, the Vero cell viability remained consistently high, namely between 91% and 96% across exposure times. These results highlight this device's potential for the surface modification of materials and various infection-related applications, boasting affordability and facilitating effective antimicrobial interventions.

Keywords: Candida albicans; Staphylococcus aureus; Vero cell; affordable cost; antimicrobial effects; cold atmospheric plasma; cytotoxicity; dielectric barrier discharge; polyethylene; polymer surface modification.

MeSH terms

  • Animals
  • Candida albicans* / drug effects
  • Chlorocebus aethiops
  • Microbial Viability / drug effects
  • Plasma Gases* / chemistry
  • Plasma Gases* / pharmacology
  • Polymers / chemistry
  • Staphylococcus aureus* / drug effects
  • Surface Properties*
  • Vero Cells

Substances

  • Plasma Gases
  • Polymers

Grants and funding

This work was supported by the São Paulo State Research Foundation (FAPESP) (grant no. 2019/05856-7), the National Council for Scientific and Technological Development (CNPq), and Coordination for the Improvement of Higher Education (CAPES).