p-Coumaric acid potential in restoring neuromotor function and oxidative balance through the Parkin pathway in a Parkinson disease-like model in Drosophila melanogaster

Kétnne Hanna Poleto; Dieniffer Espinosa Janner; Mustafa Munir Mustafa Dahleh; Márcia Rósula Poetini; Eliana Jardim Fernandes; Elize Aparecida Santos Musachio; Francielli Polet de Almeida; Elen Caroline de Matos Amador; Jocemara Corrêa Reginaldo; Murilo Ricardo Sigal Carriço; Rafael Roehrs; Marina Prigol; Gustavo Petri Guerra

doi:10.1016/j.fct.2024.115002

p-Coumaric acid potential in restoring neuromotor function and oxidative balance through the Parkin pathway in a Parkinson disease-like model in Drosophila melanogaster

Food Chem Toxicol. 2024 Nov:193:115002. doi: 10.1016/j.fct.2024.115002. Epub 2024 Sep 12.

Authors

Affiliations

¹ Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactive Molecules, Federal University of Pampa, Itaqui Campus, Itaqui, Rio Grande do Sul, Brazil; Graduate Program in Biochemistry, Federal University of Pampa, Uruguaiana Campus, Uruguaiana, Rio Grande do Sul, Brazil.
² Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactive Molecules, Federal University of Pampa, Itaqui Campus, Itaqui, Rio Grande do Sul, Brazil.
³ Graduate Program in Biochemistry, Federal University of Pampa, Uruguaiana Campus, Uruguaiana, Rio Grande do Sul, Brazil; Environmental and Toxicological Chemical Analysis Laboratory, Federal University of Pampa, Uruguaiana Campus, Uruguaiana, Rio Grande do Sul, Brazil.
⁴ Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactive Molecules, Federal University of Pampa, Itaqui Campus, Itaqui, Rio Grande do Sul, Brazil; Graduate Program in Biochemistry, Federal University of Pampa, Uruguaiana Campus, Uruguaiana, Rio Grande do Sul, Brazil. Electronic address: [email protected].

PMID: 39276910
DOI: 10.1016/j.fct.2024.115002

Abstract

p-Coumaric acid is a significant phenolic compound known for its potent antioxidant activity. Thus, this study investigated the effects of p-coumaric acid on the behavioral and neurochemical changes induced in Drosophila melanogaster by exposure to rotenone in a Parkinson disease (PD)-like model. The flies were divided into four groups and maintained for seven days on different diets: a standard diet (control), a diet containing rotenone (500 μM), a control diet to which p-coumaric acid was added on the fourth day (0.3 μM), and a diet initially containing rotenone (500 μM) with p-coumaric acid added on the fourth day (0.3 μM). Exposure to p-coumaric acid ameliorated locomotor impairment and reduced mortality induced by rotenone. Moreover, p-coumaric acid normalized oxidative stress markers (ROS, TBARS, SOD, CAT, GST, and NPSH), mitigated oxidative damage, and reflected in the recovery of dopamine levels, AChE activity, and cellular viability post-rotenone exposure. Additionally, p-coumaric acid restored the immunoreactivity of Parkin and Nrf2. The results affirm that p-coumaric acid effectively mitigates PD-like model-induced damage, underscoring its antioxidant potency and potential neuroprotective effect.

Keywords: Antioxidant; Neurodegeneration; Neuroprotective; Phenolic compound; Rotenone.

MeSH terms

Animals
Antioxidants / pharmacology
Coumaric Acids* / pharmacology
Disease Models, Animal*
Drosophila melanogaster* / drug effects
Male
Oxidative Stress* / drug effects
Parkinson Disease* / drug therapy
Parkinson Disease* / metabolism
Propionates* / pharmacology
Rotenone* / toxicity
Ubiquitin-Protein Ligases* / genetics
Ubiquitin-Protein Ligases* / metabolism

Substances

Coumaric Acids
p-coumaric acid
Propionates
Rotenone
parkin protein
Ubiquitin-Protein Ligases
Antioxidants