UnTWISTing intestinal fibrosis: single-cell transcriptomics deciphers fibroblast heterogeneity, uncovers molecular pathways, and identifies therapeutic targets

J Clin Invest. 2024 Sep 17;134(18):e184112. doi: 10.1172/JCI184112.

Abstract

Intestinal fibrosis is a severe complication of Crohn's disease, often requiring surgical intervention. Despite extensive research efforts, an effective treatment to prevent or reverse intestinal fibrosis remains elusive. In this issue of the JCI, Zhang, Wang, and colleagues employed single-cell RNA sequencing to uncover mechanisms of the fibrotic process. They identified a key fibroblast subset of TWIST1+FAP+ cells that interacts with CXCL9+ macrophages. TWIST1 emerged as a central regulator of the fibrotic microenvironment, representing a promising therapeutic target for effectively treating intestinal fibrosis.

Publication types

  • Comment

MeSH terms

  • Animals
  • Crohn Disease / genetics
  • Crohn Disease / metabolism
  • Crohn Disease / pathology
  • Fibroblasts* / metabolism
  • Fibroblasts* / pathology
  • Fibrosis*
  • Humans
  • Intestines / pathology
  • Macrophages / metabolism
  • Macrophages / pathology
  • Nuclear Proteins
  • Single-Cell Analysis*
  • Transcriptome
  • Twist-Related Protein 1* / genetics
  • Twist-Related Protein 1* / metabolism

Substances

  • Twist-Related Protein 1
  • TWIST1 protein, human
  • Nuclear Proteins