Solid Self Nano-Emulsifying Drug Delivery System of Dasatinib: Optimization, In-vitro, Ex-vivo and In-vivo assessment

Ther Deliv. 2024;15(10):749-768. doi: 10.1080/20415990.2024.2397330. Epub 2024 Sep 17.

Abstract

Aim: Dasatinib (DST) is an oral tyrosine kinase inhibitor with poor aqueous solubility. To outwit this issue, a solid self-nano emulsifying drug delivery system (S-SNEDDS) of DST was formulated.Methods: I-optimal mixture design was used for optimization of DST-loaded SNEDDS using Linalool, Cremophor RH40 and Transcutol P. S-SNEDDS underwent physicochemical characterization, in-vitro release and ex-vivo permeation, cell-based assays and pharmacokinetic study.Results: DST-S-SNEDDS showed globule size and PDI of 141.53 ± 5.371 nm and 0.282 ± 0.020, respectively. DST-S-SNEDDS revealed significantly lower IC50 (1.825 μg/mL) than free DST (7.298 μg/mL) in MDA-MB-231. In-vivo pharmacokinetic study revealed 1.94-fold increment in AUC0-t for the DST-S-SNEDDS group than free DST.Conclusion: S-SNEDDS could be promising approach for improving bioavailability and efficacy of DST.

Keywords: SNEDDS; dasatinib; dissolution; oral-bioavailability; triple-negative breast cancer.

Plain language summary

[Box: see text].

MeSH terms

  • Administration, Oral
  • Animals
  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacokinetics
  • Biological Availability*
  • Cell Line, Tumor
  • Chemistry, Pharmaceutical / methods
  • Dasatinib* / administration & dosage
  • Dasatinib* / chemistry
  • Dasatinib* / pharmacokinetics
  • Drug Delivery Systems / methods
  • Drug Liberation
  • Emulsions*
  • Ethylene Glycols / chemistry
  • Humans
  • Male
  • Nanoparticle Drug Delivery System / chemistry
  • Nanoparticles / chemistry
  • Particle Size
  • Polyethylene Glycols / chemistry
  • Protein Kinase Inhibitors / administration & dosage
  • Protein Kinase Inhibitors / chemistry
  • Protein Kinase Inhibitors / pharmacokinetics
  • Rats
  • Rats, Sprague-Dawley
  • Solubility*

Substances

  • Dasatinib
  • Emulsions
  • Polyethylene Glycols
  • Protein Kinase Inhibitors
  • Nanoparticle Drug Delivery System
  • cremophor
  • carbitol
  • Ethylene Glycols
  • Antineoplastic Agents