Objective: To establish a human buccal mucosa squamous cell carcinoma (BMSCC) cell line SCC117 in China, analyze and identify its basic biological characteristics. Methods: A 59-year-old Chinese male patient with BMSCC in the Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology in January 2011 was included in this study, his surgical specimens were primary cultured in vitro by improved tissue block culture method. The BMSCC cell line SCC117 was established after continuous passage and stable growth. The morphological characteristics of the cells were observed by light and electron microscope, and their basic biological characteristics were analyzed by growth curve, chromosome karyotype and xenotransplantation tumorigenicity in nude mice experiment. The expressions of cytokeratin (CK14), tumor-related proteins retinoblastoma tumor suppressor protein (RB), P53, E-cadherin, P21, phosphatase and tensin homolog deleted on chromosome ten (PTEN) were detected by immunohistochemical and human papilloma virus (HPV) were tested by PCR. SCC117 was identified by short tandem repeat (STR) analysis of genomic DNA. Results: SCC117, a human BMSCC cell line, had been continuously subcultured in vitro for more than 150 generations. The cells grew in polygonal mosaic and lost contact inhibition, the typical desmosomes and tensional fibrils were observed by electron microscope, and CK14 was positive by immunohistochemistry. The doubling time was 40.16 h, the chromosome mode of the cell line was concentrated between 67 and 69, hypo-triploid. All 4 nude mice inoculated with SCC117 cells developed tumors, indicating that the SCC117 cell line had the ability of xenogeneic tumorigenesis. The histopathological type of the transplanted tumor in nude mice was consistent with that of the primary tumor tissue, both of which were squamous cell carcinoma. The immunohistochemical results showed that in both human primary tumor and the transplanted tumor tissue in nude mice, RB, P53, and E-cadherin were all positive, P21 was weakly positive, while PTEN was negative. SCC117 was tested negative for the presence of HPV. STR sequence analysis showed that SCC117 cell line originated from primary tumor tissue and was not cross-contaminated by other cell lines. Conclusions: The human BMSCC cell line SCC117 was successfully established in China, which could provide a new experimental model for the study of oral SCC without HPV infection, especially BMSCC.
目的： 建立中国人颊黏膜鳞状细胞癌（BMSCC）细胞系SCC117，分析并鉴定其基本生物学特性。 方法： 纳入2011年1月就诊于北京大学口腔医学院·口腔医院口腔颌面外科的1例BMSCC患者（59岁中国男性），采用改良组织块培养法对其原发灶手术标本进行体外原代培养，连续传代稳定生长后建立人BMSCC细胞系SCC117。光学显微镜和电镜观察SCC117细胞形态特征，通过生长曲线、染色体核型、异种移植裸鼠成瘤实验等分析其基本生物学特征，免疫组化检测细胞角蛋白（CK14）、肿瘤相关蛋白视网膜母细胞瘤抑制蛋白（RB）、P53、上皮钙黏素（E-cadherin）、P21、同源性磷酸酶-张力蛋白（PTEN）表达情况，PCR检测人乳头瘤病毒（HPV）携带情况。基因组 DNA 短串联重复序列（STR）分析鉴定细胞系身份。 结果： 人BMSCC细胞系SCC117已在体外连续传代培养超过150代。SCC117细胞呈多角形镶嵌状生长，失去接触抑制，电镜观察可见典型的细胞桥粒结构及张力原纤维。免疫组化显示，SCC117细胞CK14阳性，倍增时间为40.16 h。染色体众数集中在67~69条之间，为亚三倍体。接种SCC117细胞的4只裸鼠均成瘤，SCC117细胞系具有异种致瘤能力，HE染色显示，裸鼠移植瘤与人原发肿瘤组织的病理组织学类型一致，均为鳞状细胞癌。免疫组化结果显示，人原发肿瘤及裸鼠移植瘤组织中RB、P53、E-cadherin均为阳性，P21弱阳性，PTEN阴性。PCR结果显示，SCC117细胞系的HPV检测为阴性。STR序列分析结果显示，SCC117细胞系来源于原发肿瘤组织，未被其他细胞系交叉污染。 结论： 本研究成功建立中国人BMSCC细胞系SCC117，可为无HPV感染的口腔鳞状细胞癌尤其是BMSCC的研究提供新的实验模型。.