Ergostanes from the mushroom Trametes versicolor and their cancer cell inhibition: In vitro and in silico evaluation

Steroids. 2024 Dec:212:109511. doi: 10.1016/j.steroids.2024.109511. Epub 2024 Sep 19.

Abstract

In this study, five steroid compounds were isolated from the fruiting bodies mushroom Trametes versicolor. The compounds, 9,19-cyclolanostane-3,29-diol (3), ergosta-7,22-dien-3-acetate (4), and ergosta-8(14),22-dien-3β,5α,6β,7α-tetrol (5), were identified from T. versicolor for the first time. The five compounds were evaluated for their activity against cancer cell lines. Compound 5α,8α-epidioxyergosta-6,22-dien-3β-ol (1) was found to be the most effective against most of the cancer cell lines tested. In silico studies showed that compound 1 has good binding affinities to different cancer targets, namely cyclin-dependent kinase 2 (cdk2), human cyclin-dependent kinase 6 (cdk6), Human Topo IIa ATPase/AMP-PNP, anti-apoptotic protein Bcl-2, and Vegfr-2. It's also druglike based on Lipinski's rule of five and it's ADME/Tox properties. Therefore, compound 1 is a good candidate in the management of cancer. These results further show that T. versicolor is a potential source of drugs or drug leads for cancer treatment.

Keywords: ADMET profiling; Anticancer; Molecular docking; Mushroom; T. versicolor.

Publication types

  • Review

MeSH terms

  • Antineoplastic Agents* / chemistry
  • Antineoplastic Agents* / isolation & purification
  • Antineoplastic Agents* / pharmacology
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Computer Simulation
  • Drug Screening Assays, Antitumor
  • Ergosterol* / analogs & derivatives
  • Ergosterol* / chemistry
  • Ergosterol* / isolation & purification
  • Ergosterol* / pharmacology
  • Humans
  • Molecular Docking Simulation
  • Polyporaceae / chemistry

Substances

  • Antineoplastic Agents
  • Ergosterol
  • ergostane

Supplementary concepts

  • Trametes versicolor