Regulatory role of lncMD1 in goat skeletal muscle satellite cell differentiation via miR-133a-3p and miR-361-3p targeting

Int J Biol Macromol. 2024 Nov;280(Pt 2):135807. doi: 10.1016/j.ijbiomac.2024.135807. Epub 2024 Sep 19.

Abstract

Skeletal muscle satellite cells (SMSCs) are pivotal in skeletal muscle development and are influenced by numerous regulatory factors. This study focuses on the regulatory and functional mechanism roles of lncMD1, a muscle-specific long non-coding RNA, in the proliferation and differentiation of goat SMSCs. Employing in vitro cultured goat SMSCs, this study demonstrated that lncMD1, functions as a decoy for miR-133a-3p and miR-361-3p. This interaction competitively binds these microRNAs to modulate the expression of dynactin subunit 2 (DCTN2) and dynactin subunit 1 (DCTN1), thereby affects SMSCs proliferation and differentiation. These findings enhance the understanding of non-coding RNAs in goat SMSCs growth cycles and offer a theoretical foundation for exploring the molecular processes of goat skeletal muscle myogenic development.

Keywords: Goat; Muscle development; Skeletal muscle satellite cell; lncRNA; miRNA.

MeSH terms

  • Animals
  • Cell Differentiation* / genetics
  • Cell Proliferation* / genetics
  • Cells, Cultured
  • Gene Expression Regulation
  • Goats* / genetics
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Muscle Development / genetics
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • Satellite Cells, Skeletal Muscle* / cytology
  • Satellite Cells, Skeletal Muscle* / metabolism

Substances

  • MicroRNAs
  • RNA, Long Noncoding