Phytochemical Investigation of an Ostrya carpinifolia L. Extract: An Effective Anti-Pollution Cosmetic Active Ingredient

Chem Biodivers. 2025 Jan;22(1):e202402139. doi: 10.1002/cbdv.202402139. Epub 2024 Nov 7.

Abstract

Ostrya carpinifolia L., a member of the Betulaceae family, is a tree endemic to the Mediterranean basin that is well known for the hardness of its wood. In this study, we assess the anti-pollution activities of a hydroalcoholic extract of O. carpinifolia twigs using several judiciously selected in vitro cosmetic bioassays. The extract's capacity to counteract excessive production of reactive oxygen species following a cutaneous exposure to atmospheric pollution was evaluated using a combination of several antioxidant assays: DPPH, FRAP and β-carotene bleaching assays. These antioxidant assays were complemented by anti-elastase, anti-collagenase, anti-hyaluronidase and anti-lipoxygenase assays to evaluate the capacity of the extract to preserve the integrity of the skin. The hydroalcoholic extract of O. carpinifolia demonstrates intriguing biological antioxidant activities, with approximately 50 % inhibition observed in DPPH and β-carotene assays. Furthermore, its anti-lipoxygenase, anti-hyaluronidase, and anti-collagenase activities are noteworthy, exceeding 50 % inhibition. The two major compounds of O. carpinifolia ethanolic extract were isolated and identified as myricitrin (1) and quercitrin (2). Myricitrin and quercitrin exhibit antioxidant and anti-hyaluronidase properties; we explored the correlation of these properties with the activity of the crude hydroalcoholic extract. Notably, these compounds have not been previously described in the Ostrya genus.

Keywords: Anti-pollution activity; Cosmetic bioassays; Flavonoids; Ostrya carpinifolia L.; Phenolic compounds.

MeSH terms

  • Animals
  • Antioxidants* / chemistry
  • Antioxidants* / isolation & purification
  • Antioxidants* / pharmacology
  • Biphenyl Compounds / antagonists & inhibitors
  • Collagenases / metabolism
  • Cosmetics* / chemistry
  • Cosmetics* / pharmacology
  • Humans
  • Hyaluronoglucosaminidase / antagonists & inhibitors
  • Hyaluronoglucosaminidase / metabolism
  • Pancreatic Elastase / antagonists & inhibitors
  • Pancreatic Elastase / metabolism
  • Phytochemicals* / chemistry
  • Phytochemicals* / isolation & purification
  • Phytochemicals* / pharmacology
  • Picrates / antagonists & inhibitors
  • Plant Extracts* / chemistry
  • Plant Extracts* / isolation & purification
  • Plant Extracts* / pharmacology
  • Quercetin / chemistry
  • Quercetin / isolation & purification
  • Quercetin / pharmacology
  • Reactive Oxygen Species / metabolism
  • beta Carotene / chemistry

Substances

  • Plant Extracts
  • Antioxidants
  • Cosmetics
  • Phytochemicals
  • Picrates
  • Biphenyl Compounds
  • 1,1-diphenyl-2-picrylhydrazyl
  • Hyaluronoglucosaminidase
  • Collagenases
  • beta Carotene
  • Reactive Oxygen Species
  • Quercetin
  • Pancreatic Elastase