Mineralo-organic particles inhibit influenza A virus infection by targeting viral hemagglutinin activity

Nanomedicine (Lond). 2024;19(28):2375-2390. doi: 10.1080/17435889.2024.2403326. Epub 2024 Sep 25.

Abstract

Aim: Mineralo-organic particles, naturally present in human body fluids, participate in ectopic calcification and inflammatory diseases. These particles coexist with influenza A virus (IAV) in the same microenvironment during viral infection. Our objective was to investigate the functional consequences of the potential interactions between these particles and the virions.Materials & methods: We used in vitro models, including electron microscopy, fluorescence microscopy, hemagglutination assay and viral infection assays to examine the interactions.Results: Mineralo-organic particles bind to IAV virions through interactions involving particle-bound fetuin-A and mineral content, effectively engaging viral hemagglutinin. These interactions result in hindered viral infection.Conclusion: These findings uncover the novel interactions between mineralo-organic particles and IAV, highlighting the impact of virus microenvironment complexity.

Keywords: fetuin-A; hemagglutination; hemagglutinin; infectious diseases; influenza A virus; mineral particles; mineralo-organic particles.

Plain language summary

[Box: see text].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / chemistry
  • Antiviral Agents / pharmacology
  • Dogs
  • Hemagglutinin Glycoproteins, Influenza Virus* / chemistry
  • Hemagglutinin Glycoproteins, Influenza Virus* / metabolism
  • Humans
  • Influenza A virus* / drug effects
  • Influenza, Human* / virology
  • Madin Darby Canine Kidney Cells
  • Virion / metabolism

Substances

  • Hemagglutinin Glycoproteins, Influenza Virus
  • Antiviral Agents