Innovative approach of nanopore-based metagenomic sequencing for rapid identification of bacterial pathogens in joint fluid: A pilot study

Diagn Microbiol Infect Dis. 2024 Dec;110(4):116545. doi: 10.1016/j.diagmicrobio.2024.116545. Epub 2024 Sep 23.

Abstract

Analysis of 11 clinical samples of joint fluid in this pilot study demonstrated proof-of-concept for nanopore-based metagenomic sequencing to serve as a complementary real-time diagnostic technique for septic arthritis, with a sensitivity of 75.0 % and specificity of 57.1 %, compared to the gold standard method of bacterial culture.

Keywords: Infectious arthritis; Nanopore sequencing; Point-of-care testing; Synovial fluid.

MeSH terms

  • Arthritis, Infectious* / diagnosis
  • Arthritis, Infectious* / microbiology
  • Bacteria* / classification
  • Bacteria* / genetics
  • Bacteria* / isolation & purification
  • High-Throughput Nucleotide Sequencing / methods
  • Humans
  • Metagenomics* / methods
  • Nanopore Sequencing* / methods
  • Nanopores
  • Pilot Projects
  • Sensitivity and Specificity
  • Synovial Fluid / microbiology