Cellulose / waste Cu2+-activated carbon composite: A sustainable and green material for boosting laccase activity and degradation of bisphenol A in wastewater

Na Li; Fangrui Yu; Hongbin Li; Xiangrui Meng; Chuanbo Peng; Xueru Sheng; Jian Zhang; Shanshan Liu; Qingwei Ping; Huining Xiao

doi:10.1016/j.ijbiomac.2024.136121

Cellulose / waste Cu²⁺-activated carbon composite: A sustainable and green material for boosting laccase activity and degradation of bisphenol A in wastewater

Int J Biol Macromol. 2024 Nov;281(Pt 1):136121. doi: 10.1016/j.ijbiomac.2024.136121. Epub 2024 Sep 27.

Authors

Na Li¹, Fangrui Yu², Hongbin Li³, Xiangrui Meng⁴, Chuanbo Peng⁵, Xueru Sheng⁶, Jian Zhang⁷, Shanshan Liu⁸, Qingwei Ping⁹, Huining Xiao¹⁰

Affiliations

¹ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; Liaoning Province Key Laboratory of Pulp and Papermaking Engineering, Dalian Polytechnic University, Dalian l16034, China. Electronic address: [email protected].
² Liaoning Province Key Laboratory of Pulp and Papermaking Engineering, Dalian Polytechnic University, Dalian l16034, China. Electronic address: [email protected].
³ Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Jiangsu Province Key Laboratory of Biomass Energy and Materials, Nanjing 210042, China. Electronic address: [email protected].
⁴ Liaoning Province Key Laboratory of Pulp and Papermaking Engineering, Dalian Polytechnic University, Dalian l16034, China. Electronic address: [email protected].
⁵ Liaoning Province Key Laboratory of Pulp and Papermaking Engineering, Dalian Polytechnic University, Dalian l16034, China. Electronic address: [email protected].
⁶ Liaoning Province Key Laboratory of Pulp and Papermaking Engineering, Dalian Polytechnic University, Dalian l16034, China. Electronic address: [email protected].
⁷ Liaoning Province Key Laboratory of Pulp and Papermaking Engineering, Dalian Polytechnic University, Dalian l16034, China. Electronic address: [email protected].
⁸ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China. Electronic address: [email protected].
⁹ Liaoning Province Key Laboratory of Pulp and Papermaking Engineering, Dalian Polytechnic University, Dalian l16034, China. Electronic address: [email protected].
¹⁰ Department of Chemical Engineering, University of New Brunswick, Fredericton, NB E3B5A3, Canada. Electronic address: [email protected].

PMID: 39343265
DOI: 10.1016/j.ijbiomac.2024.136121

Abstract

Low enzyme activity is one of the disadvantages of immobilized laccase. In this study, waste Cu²⁺-loaded activated carbon (Cu-AC) was successfully used in preparing a novel composite support，cellulose / Cu²⁺-loaded activated carbon beads (C / Cu-AC)， and effectively boosted immobilized laccase activity. To achieve optimum conditions for immobilization of laccase, the immobilization time, pH and laccase concentration were examined. The highest immobilized laccase activity (34.21 U/g) was achieved under optimum conditions (T = 4 h, pH = 4, C = 5 g/L), which was increased by 35.86 % compared to control. In addition, the immobilized laccase showed an outstanding performance in thermostability and reusability compared to free laccase. Moreover, the degradation of BPA by immobilized laccase was carried out, and the optimum degradation conditions were explored. Under such conditions: concentration of BPA was 75 mg / L and pH = 4, t = 1 h, T = 50 °C，the removal yield of BPA reached a maximum of 79.88 %. Therefore, the utilization of waste Cu-CA is a powerful method to boost immobilized laccase activity and creating a new way to high value treatment of waste Cu-CA.

Keywords: Biomass; Enzyme activity; Immobilized laccase.

MeSH terms

Benzhydryl Compounds* / chemistry
Cellulose* / chemistry
Charcoal* / chemistry
Copper* / chemistry
Enzyme Stability
Enzymes, Immobilized* / chemistry
Enzymes, Immobilized* / metabolism
Hydrogen-Ion Concentration
Laccase* / chemistry
Laccase* / metabolism
Phenols* / chemistry
Temperature
Wastewater* / chemistry
Water Pollutants, Chemical / chemistry
Water Purification / methods

Substances

Laccase
Benzhydryl Compounds
bisphenol A
Phenols
Wastewater
Enzymes, Immobilized
Copper
Cellulose
Charcoal
Water Pollutants, Chemical