Flexible and robust cell type annotation for highly multiplexed tissue images

bioRxiv [Preprint]. 2024 Dec 17:2024.09.12.612510. doi: 10.1101/2024.09.12.612510.

Abstract

Identifying cell types in highly multiplexed images is essential for understanding tissue spatial organization. Current cell type annotation methods often rely on extensive reference images and manual adjustments. In this work, we present a tool, Robust Image-Based Cell Annotator (RIBCA), that enables accurate, automated, unbiased, and fine-grained cell type annotation for images with a wide range of antibody panels, without requiring additional model training or human intervention. Our tool has successfully annotated over 3 million cells, revealing the spatial organization of various cell types across more than 40 different human tissues. It is open-source and features a modular design, allowing for easy extension to additional cell types.

Publication types

  • Preprint