Targeted isolation of lignans from the roots of Anthriscus sylvestris (L.) Hoffm. by small molecule accurate recognition technology

Yan-Ling Liu; Yan-Gang Cao; Ya-Juan Zheng; Ying Niu; Lan Chen; Xu Chen; Xin-Yi Ma; Xiang-da Li; Xiao-Ke Zheng; Wei-Sheng Feng

doi:10.1016/j.phytochem.2024.114293

Targeted isolation of lignans from the roots of Anthriscus sylvestris (L.) Hoffm. by small molecule accurate recognition technology

Phytochemistry. 2025 Jan:229:114293. doi: 10.1016/j.phytochem.2024.114293. Epub 2024 Sep 28.

Authors

Affiliations

¹ School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China.
² School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China; Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Disease Diagnosis by Henan and Education Ministry of PR China, Zhengzhou, 450046, China. Electronic address: [email protected].
³ School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China; Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Disease Diagnosis by Henan and Education Ministry of PR China, Zhengzhou, 450046, China. Electronic address: [email protected].

PMID: 39349233
DOI: 10.1016/j.phytochem.2024.114293

Abstract

Five undescribed lignans (1-5), along with sixteen known lignans (6-21), were isolated from the roots of Anthriscus sylvestris using small molecule accurate recognition technology (SMART). The structures of the isolated compounds were determined by comprehensive spectroscopic analyses, and the absolute configurations of compounds 3-5 were elucidated by comparison of their calculated and experimental ECD spectra. Compounds 5, 14-15, 19, and 21 exhibited significantly inhibitory effects against hypoxia-stimulated abnormal proliferation of pulmonary arterial smooth muscle cells (PASMCs). Moreover, compounds 5, 14-15, 19, and 21 can significantly restore expression of expression of PASMCs proliferation-related protein, including α-SMA, PCNA, P27, and CyclinD3, which are closely related to cell proliferation.

Keywords: Anthriscus sylvestris; Anti-proliferative effect; Apiaceae; Lignans; NMR spectra-guided isolation.

MeSH terms

Animals
Apiaceae* / chemistry
Cell Proliferation* / drug effects
Dose-Response Relationship, Drug
Lignans* / chemistry
Lignans* / isolation & purification
Lignans* / pharmacology
Molecular Structure
Myocytes, Smooth Muscle / drug effects
Myocytes, Smooth Muscle / metabolism
Plant Roots* / chemistry
Small Molecule Libraries / chemistry
Small Molecule Libraries / isolation & purification
Small Molecule Libraries / pharmacology

Substances

Lignans
Small Molecule Libraries