Sensitivity, Specificity, and Cost-Benefit Effect Between Primary Human Papillomavirus Testing, Primary Liquid-Based Cytology, and Co-Testing Algorithms for Cervical Lesions

Diagn Cytopathol. 2025 Jan;53(1):35-42. doi: 10.1002/dc.25410. Epub 2024 Oct 9.

Abstract

Background: Cytology has long been a major screening method for cervical cancer prevention. Human papillomavirus (HPV) testing has recently been introduced for cervical cancer screening, and HPV tests become a major screening method in some countries. To seek the optimal strategy considering the cost-effectiveness for cervical cancer screening, we compared the performance of primary LBC, primary HPV test, and LBC plus HPV co-test in real practice.

Methods: From March 2016 to June 2018, 3742 patients were included in this study. Liquid-based cytology (LBC), HPV test, and histopathological assessment were performed in 3727, 1063, and 508 cases, respectively. The sensitivity, specificity, and cost-benefit effects of primary HPV, primary LBC, and co-test algorithms were simulated for 317 cases with LBC, HPV, and histopathological results.

Results: On the LBC, 13.0% of the cases were diagnosed with atypical squamous cells of undetermined significance or higher grade lesions. In the HPV test, high-risk HPV was found in 43.5%, and 11.9% was positive for HPV type 16 or 18. Among the three simulated algorithms, the co-test demonstrated the best sensitivity (97.5%) and the lowest specificity (50.3%). The primary LBC demonstrated the best specificity (53.5%) and a slightly better sensitivity, compare with the primary HPV (95.1% vs. 93.8%). Using the primary LBC algorithm, 82.0% can be determined without additional HPV test, whereas 50.1% could be determined without additional LBC using the primary HPV algorithm.

Conclusions: The primary LBC algorithm for uterine cervical cancer (UCC) screening is comparable to the primary HPV algorithm and has the best cost-benefit effect among the three algorithms.

Keywords: cytology; human papillomavirus; pathology; uterine cervical cancer.

MeSH terms

  • Adult
  • Aged
  • Algorithms*
  • Cost-Benefit Analysis*
  • Cytodiagnosis / economics
  • Cytodiagnosis / methods
  • Early Detection of Cancer / economics
  • Early Detection of Cancer / methods
  • Female
  • Human Papillomavirus Viruses
  • Humans
  • Liquid Biopsy / economics
  • Liquid Biopsy / methods
  • Middle Aged
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections* / diagnosis
  • Papillomavirus Infections* / pathology
  • Sensitivity and Specificity*
  • Uterine Cervical Neoplasms* / diagnosis
  • Uterine Cervical Neoplasms* / pathology
  • Uterine Cervical Neoplasms* / virology
  • Vaginal Smears / economics
  • Vaginal Smears / methods