Neutralizing chimeric anti-F1 monoclonal antibody against Yersinia pestis infection

Int J Antimicrob Agents. 2024 Dec;64(6):107354. doi: 10.1016/j.ijantimicag.2024.107354. Epub 2024 Oct 9.

Abstract

Drug-resistant Yersinia pestis (Y. pestis) poses a threat to the use of antibiotics to treat Y. pestis infections. Passive immunization with neutralizing monoclonal antibodies (mAbs) is considered an effective approach for the treatment of infectious diseases. In this study, a murine single-chain fragment variable (scFv) phage antibody library targeting the F1 antigen was constructed and screened. Therapeutic intravenous injection of 400 μg chimeric mAb S1 through tail veins provided complete protection against Y. pestis 201 challenge in a pneumonic plague mouse model. Timely antibody treatment eliminated the bacteria and reduced lung inflammation. These data suggest that chimeric mAb S1 is a candidate treatment for Y. pestis infection that warrants further study.

Keywords: Chimeric monoclonal antibody; Phage display; Pneumonic plague; Yersinia pestis.

MeSH terms

  • Animals
  • Antibodies, Bacterial / immunology
  • Antibodies, Bacterial / therapeutic use
  • Antibodies, Monoclonal* / immunology
  • Antibodies, Monoclonal* / therapeutic use
  • Antibodies, Neutralizing* / immunology
  • Antibodies, Neutralizing* / therapeutic use
  • Bacterial Proteins / immunology
  • Disease Models, Animal*
  • Female
  • Immunization, Passive / methods
  • Injections, Intravenous
  • Mice
  • Mice, Inbred BALB C
  • Plague* / immunology
  • Plague* / prevention & control
  • Single-Chain Antibodies / immunology
  • Single-Chain Antibodies / therapeutic use
  • Yersinia pestis* / immunology

Substances

  • Antibodies, Neutralizing
  • Antibodies, Monoclonal
  • Antibodies, Bacterial
  • caf1 protein, Yersinia pestis
  • Single-Chain Antibodies
  • Bacterial Proteins