Exploring glutathione transferase and Cathepsin L-like proteinase for designing of epitopes-based vaccine against Fasciola hepatica by immunoinformatics and biophysics studies

Front Immunol. 2024 Sep 26:15:1478107. doi: 10.3389/fimmu.2024.1478107. eCollection 2024.

Abstract

Fasciolosis is a zoonotic infection and is considered a developing deserted tropical illness threatening ruminant productivity and causing financial losses. Herein, we applied immunoinformatics and biophysics studies to develop an epitopes vaccine against Fasciola hepatica using glutathione transferase and Cathepsin L-like proteinase as possible vaccine candidates. Using the selected proteins, B- and T-cell epitopes were predicted. After epitopes prediction, the epitopes were clarified over immunoinformatics screening, and only five epitopes, EFGRWQQEKCTIDLD, RRNIWEKNVKHIQEH, FKAKYLTEMSRASDI, TDMTFEEFKAKYLTE, and YTAVEGQCR were selected for vaccine construction; selected epitopes were linked with the help of a GPGPG linker and attached with an adjuvant through another linker, EAAAK linker. Cholera toxin B subunit was used as an adjuvant. The ExPASy ProtParam tool server predicted 234 amino acids, 25.86257 kDa molecular weight, 8.54 theoretical pI, 36.86 instability index, and -0.424 grand average of hydropathicity. Molecular docking analysis predicted that the vaccine could activate the immune system against F. hepatica. We calculated negative binding energy values. A biophysics study, likely molecular docking molecular dynamic simulation, further validated the docking results. In molecular dynamic simulation analysis, the top hit docked compounds with the lowest binding energy values were subjected to MD simulation; the simulation analysis showed that the vaccine and immune cell receptors are stable and can activate the immune system. MMGBSA of -146.27 net energy (kcal/mol) was calculated for the vaccine-TLR2 complex, while vaccine-TLR4 of -148.11 net energy (kcal/mol) was estimated. Furthermore, the C-ImmSim bioinformatics tool predicted that the vaccine construct can activate the immune system against F. hepatica, eradicate the infection caused by F. hepatica, and reduce financial losses that need to be spent while protecting against infections of F. hepatica. The computational immune simulation unveils that the vaccine model can activate the immune system against F. hepatica; hence, the experimental scientist can validate the finding accomplished through computational approaches.

Keywords: Fasciola hepatica; epitopes; immunoinformatics; molecular docking; molecular dynamic simulation analysis.

MeSH terms

  • Animals
  • Antigens, Helminth / chemistry
  • Antigens, Helminth / immunology
  • Cathepsin L / immunology
  • Computational Biology* / methods
  • Epitopes, B-Lymphocyte* / immunology
  • Epitopes, T-Lymphocyte* / immunology
  • Fasciola hepatica* / enzymology
  • Fasciola hepatica* / immunology
  • Fascioliasis* / immunology
  • Fascioliasis* / parasitology
  • Fascioliasis* / prevention & control
  • Glutathione Transferase* / chemistry
  • Glutathione Transferase* / genetics
  • Glutathione Transferase* / immunology
  • Helminth Proteins / chemistry
  • Helminth Proteins / immunology
  • Immunoinformatics
  • Molecular Docking Simulation*
  • Molecular Dynamics Simulation
  • Vaccines* / immunology

Substances

  • Epitopes, T-Lymphocyte
  • Glutathione Transferase
  • Vaccines
  • Epitopes, B-Lymphocyte
  • Cathepsin L
  • Antigens, Helminth
  • Helminth Proteins

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research is funded by the Deanship of Graduate Studies and Scientific Research at Jouf University (grant number DSR2022-NF-10).