Facile synthesis of EGCG modified Au nanoparticles and their inhibitory effects on amyloid protein aggregation

Ting Wu; Yufei Zhang; Haolin Li; Zian Pan; Jianwei Ding; Wei Zhang; Shuangfei Cai; Rong Yang

doi:10.1016/j.ijbiomac.2024.136501

Facile synthesis of EGCG modified Au nanoparticles and their inhibitory effects on amyloid protein aggregation

Int J Biol Macromol. 2024 Nov;281(Pt 4):136501. doi: 10.1016/j.ijbiomac.2024.136501. Epub 2024 Oct 10.

Authors

Ting Wu¹, Yufei Zhang², Haolin Li², Zian Pan², Jianwei Ding¹, Wei Zhang³, Shuangfei Cai⁴, Rong Yang⁵

Affiliations

¹ CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China.
² CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China; Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100049, China.
³ National Key Laboratory of Computational Physics, Institute of Applied Physics and Computational Mathematics, Beijing 100088, China. Electronic address: [email protected].
⁴ CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China. Electronic address: [email protected].
⁵ CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China; Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: [email protected].

PMID: 39393717
DOI: 10.1016/j.ijbiomac.2024.136501

Abstract

Preventing β-amyloid (Aβ) peptide aggregation by Au nanoparticles (NPs) is a promising strategy for the treatment of Alzheimer's disease. However, construction of Au nanostructures with easy preparation and high therapeutic efficiency is still a challenge. Herein, one-step pulsed laser ablation in water is used to fabricate epigallocatechin-3-gallate (EGCG) modified Au (Au-EGCG) NPs with uniform size. The as-obtained Au-EGCG NPs can effectively inhibit β-amyloid (1-42) peptide (Aβ₄₂) aggregation by the interaction with peptides, which is confirmed by transmission electron microscopy (TEM), fluorescence spectroscopy (thioflavin T (ThT), tyrosine and 8-anilinonaphthalene-1-sulfonic acid (ANS) assays), and Fourier transform infrared (FT-IR) spectroscopy. Besides, they can also effectively attenuate Aβ₄₂-induced cytotoxicity based on the cell viability experiments. This work provides a facile approach to synthesize the surface-functionalized Au NPs for enhanced inhibition of Aβ aggregation and amelioration of Aβ-induced cytotoxicity.

Keywords: Alzheimer's disease; Amyloid protein; Au nanoparticles; Epigallocatechin-3-gallate; Pulsed laser ablation.

MeSH terms

Amyloid beta-Peptides* / antagonists & inhibitors
Amyloid beta-Peptides* / chemistry
Amyloid beta-Peptides* / metabolism
Catechin* / analogs & derivatives
Catechin* / chemical synthesis
Catechin* / chemistry
Catechin* / pharmacology
Cell Survival / drug effects
Gold* / chemistry
Humans
Metal Nanoparticles* / chemistry
Peptide Fragments / chemistry
Peptide Fragments / pharmacology
Protein Aggregates / drug effects

Substances

Gold
Catechin
epigallocatechin gallate
Amyloid beta-Peptides
Protein Aggregates
amyloid beta-protein (1-42)
Peptide Fragments