Co-Delivery of VEGF siRNA and THPP via Metal-Organic Framework Reverses Cisplatin-Resistant Non-Small Cell Lung Cancer and Inhibits Metastasis through a MUC4 Regulating Mechanism

ACS Appl Mater Interfaces. 2024 Oct 23;16(42):56910-56925. doi: 10.1021/acsami.4c15175. Epub 2024 Oct 14.

Abstract

Cisplatin resistance significantly impacts the antitumor efficacy of cisplatin chemotherapy and contributes to poor prognosis, including metastasis. In this study, we present the utilization of metal-organic framework (MOF) nanoparticles as the therapeutic component and drug loading scaffold for implementing a ternary combination therapeutic strategy to combat cisplatin-resistant lung cancer and metastasis. Specifically, by engineering MOFs (Cis@MOF-siVEGF) through the self-assembly of THPP as photosensitizer for photodynamic therapy (PDT), along with the incorporation of cisplatin (DDP) and VEGF siRNA (siVEGF), we propose the leverage of photodynamic-induced oxidative damage and gene silencing of the angiogenic factor to reverse cisplatin resistance and sensitize therapeutic potency. Our findings demonstrated that the chemo/photodynamic/antiangiogenic triple combination therapy via Cis@MOF-siVEGF under irradiation effectively inhibits cisplatin-resistant tumor growth and induces abscopal effects. Importantly, molecular mechanistic exploration suggested that MUC4 exerted regulatory effects on governing cancer metastasis, thus representing a potential immunotherapeutic target for cancer intervention. Overall, our study creates a MOFs-based multicomponent delivery platform for complementary therapeutic modules with synergistically enhanced antitumor efficacy and sheds light on potential regulatory mechanisms on cisplatin-resistance cancers.

Keywords: MOFs; MUC4; PDT-combined therapy; cisplatin resistance; metastasis.

MeSH terms

  • Animals
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Carcinoma, Non-Small-Cell Lung* / drug therapy
  • Carcinoma, Non-Small-Cell Lung* / pathology
  • Carcinoma, Non-Small-Cell Lung* / therapy
  • Cell Line, Tumor
  • Cisplatin* / chemistry
  • Cisplatin* / pharmacology
  • Drug Resistance, Neoplasm* / drug effects
  • Humans
  • Lung Neoplasms* / drug therapy
  • Lung Neoplasms* / pathology
  • Lung Neoplasms* / therapy
  • Metal-Organic Frameworks* / chemistry
  • Metal-Organic Frameworks* / pharmacology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Mucin-4 / metabolism
  • Neoplasm Metastasis
  • Photochemotherapy
  • Photosensitizing Agents / chemistry
  • Photosensitizing Agents / pharmacology
  • Photosensitizing Agents / therapeutic use
  • RNA, Small Interfering* / chemistry
  • RNA, Small Interfering* / pharmacology
  • Vascular Endothelial Growth Factor A* / metabolism

Substances

  • Cisplatin
  • Metal-Organic Frameworks
  • RNA, Small Interfering
  • Vascular Endothelial Growth Factor A
  • Mucin-4
  • Antineoplastic Agents
  • Photosensitizing Agents