Magnetic nanobead assisted the dual targets driven fluorescent biosensor based on SPEXPAR and MNAzyme for the olfactory marker protein detection

Jing Qi; Xuemin Cao; Hongyi Bao; Tuodi Zhang; Yichen Wang; Ya Wen; Junling Yang; Guixuan Ge; Ping Wang; Lin Chen; Feng Wang

doi:10.1016/j.mtbio.2024.101272

Magnetic nanobead assisted the dual targets driven fluorescent biosensor based on SPEXPAR and MNAzyme for the olfactory marker protein detection

Mater Today Bio. 2024 Sep 27:29:101272. doi: 10.1016/j.mtbio.2024.101272. eCollection 2024 Dec.

Authors

Jing Qi¹, Xuemin Cao¹, Hongyi Bao², Tuodi Zhang¹, Yichen Wang³, Ya Wen¹, Junling Yang¹, Guixuan Ge¹, Ping Wang⁴, Lin Chen⁵, Feng Wang^{1

2}

Affiliations

¹ Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China.
² Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China.
³ Ulink High School of Suzhou Industrial Park, Suzhou, 215128, China.
⁴ Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
⁵ Nantong Institute of Liver Diseases, Nantong Third People's Hospital Affiliated Nantong Hospital 3 of Nantong University, Nantong, 226006, China.

Abstract

Fig. 1: Schematic illustration of the principle of the Dual targets driven SPEXPAR assisted MNAzyme (D-S-M) fluorescent biosensor for olfactory marker protein (OMP) detection. A. Workflow for detection of OMP in nasal swab. B. Isothermal Self-Primer EXPonential Amplification Reaction (SPEXPAR) amplification. C. The production of fluorescent signal by Multicomponent Nuclear Acid Enzyme (MNAzyme). The signal of OMP was amplified and changed into the detectable fluorescence signal based on the reactions of SPEXPAR and MNAzyme in the D-S-M fluorescence biosensor. The qualitative or quantitative analysis of OMP can be measured by the analysis of the fluorescence intensity.Image 1.