LncRNA lncLLM Facilitates Lipid Deposition by Promoting the Ubiquitination of MYH9 in Chicken LMH Cells

Int J Mol Sci. 2024 Sep 25;25(19):10316. doi: 10.3390/ijms251910316.

Abstract

The liver plays an important role in regulating lipid metabolism in animals. This study investigated the function and mechanism of lncLLM in liver lipid metabolism in hens at the peak of egg production. The effect of lncLLM on intracellular lipid content in LMH cells was evaluated by qPCR, Oil Red O staining, and detection of triglyceride (TG) and cholesterol (TC) content. The interaction between lncLLM and MYH9 was confirmed by RNA purification chromatin fractionation (CHIRP) and RNA immunoprecipitation (RIP) analysis. The results showed that lncLLM increased the intracellular content of TG and TC and promoted the expression of genes related to lipid synthesis. It was further found that lncLLM had a negative regulatory effect on the expression level of MYH9 protein in LMH cells. The intracellular TG and TC content of MYH9 knockdown cells increased, and the expression of genes related to lipid decomposition was significantly reduced. In addition, this study confirmed that the role of lncLLM is at least partly through mediating the ubiquitination of MYH9 protein to accelerate the degradation of MYH9 protein. This discovery provides a new molecular target for improving egg-laying performance in hens and treating fatty liver disease in humans.

Keywords: MYH9; fatty liver; lipid metabolism; lncRNA; ubiquitination.

MeSH terms

  • Animals
  • Cell Line
  • Chickens*
  • Cholesterol / metabolism
  • Lipid Metabolism*
  • Liver / metabolism
  • Myosin Heavy Chains* / genetics
  • Myosin Heavy Chains* / metabolism
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • Triglycerides / metabolism
  • Ubiquitination*

Substances

  • Myosin Heavy Chains
  • RNA, Long Noncoding
  • Triglycerides
  • Cholesterol

Grants and funding

This research was funded by the National Natural Science Foundation of China (NO. 32372871), Major Scientific and Technological Special Project of Henan Province (NO. 221100110200) and National Key Research and Development Program (2022YFF1000202).