Ultrasensitive aflatoxin B1 detection based on vertical organic electrochemical transistor

Herein, we presented an ultrasensitive Aflatoxin B1 (AFB1) detection platform based on vertical organic electrochemical transistor (vOECT) first time. Chitosan-graphene nanosheets nanocomposites and AFB1 antibodies were modified on commercial electrodes as immunosensors, in series with gate electrodes of vOECT, operated at enhancement mode with ultrahigh transconductance g_m 94 mS to amplify current signals. When AFB1 is added, the impedance of the immunosensors increased due to antigen-antibody immune binding, resulting in a potential decrease in reaction cell. Then, the potential decrease leads to an effective gate voltage V_G^eff increase, contributing to a significant drain-source current I_DS decrease as a consequence of ultrahigh gm of vOECT. As a result, the presented vOECT platform exhibited an ultrahigh sensitivity of ∼1 mA/dec, and an ultralow detection limit of 0.01 fg/mL (S/N = 3), superior to all previous reported values. Furthermore, the platform exhibited satisfactory stability and specificity, and was applied to detect AFB1 in corn samples.