Use of Proximity Ligation Assay to Study Lymphoid Malignancies

Kristina Rinaldi; Arnold Bolomsky; Jan Wisniewski; Lin Zhang; Ryan M Young

doi:10.1007/978-1-0716-4188-0_12

Use of Proximity Ligation Assay to Study Lymphoid Malignancies

Methods Mol Biol. 2025:2865:273-282. doi: 10.1007/978-1-0716-4188-0_12.

Authors

Kristina Rinaldi¹, Arnold Bolomsky¹, Jan Wisniewski², Lin Zhang¹, Ryan M Young³

Affiliations

¹ Lymphoid Malignancies Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
² Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
³ Lymphoid Malignancies Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. [email protected].

PMID: 39424729
DOI: 10.1007/978-1-0716-4188-0_12

Abstract

The advent of high-throughput and unbiased proteogenomic screens promises to rapidly advance our understanding of the molecular mechanisms underpinning pathogenesis of lymphoid malignancies. The wealth of data generated from these studies requires methods to rapidly confirm and extend findings into cell line models and primary patient samples. The proximity ligation assay (PLA) is a method that can visualize protein-protein interactions in situ. PLA can capture transient interactions and characterize constituents of stable biomolecular condensates, both of which pose technical difficulties for traditional biochemical and fluorescence imaging techniques.

Keywords: Fluorescence imaging; PLA; Proximity ligation assay; Signal transduction.

MeSH terms

Cell Line, Tumor
Humans
Lymphoma / genetics
Lymphoma / metabolism
Lymphoma / pathology
Protein Binding
Protein Interaction Mapping* / methods