Tyramine-assisted fluorescent labeling of polysaccharides: Characterization, and interfacial properties

Fluorescence labeling has been widely used in various fields, including fluorescent sensors, biochemistry, medical and chemical research. This study proposed an efficient strategy for the detection and microanalysis of polysaccharides. Soy hull polysaccharide (SHP) was successfully labeled with fluorescein isothiocyanate (FITC) via an amination reaction using tyramine as a linker. The labeled polysaccharide (FTSHP) was characterized by fluorescence, UV-visible, flourier transform infrared (FT-IR), ¹H NMR, differential scanning calorimetry (DSC) and interfacial tension. The results indicated that the labeling efficiency of FTSHP with different concentration of FITC (0.20-0.35 wt%) was 1.51 %, 1.58 %, 1.63 %, and 1.67 %, respectively, with fluorescence intensity increasing as FITC concentration increased. Moreover, the interfacial adsorption capacity and thermal stability of FTSHP were significantly improved compared to SHP. This labeling method offers a promising approach for detection and analysis of polysaccharides, providing new insights into the study of other natural polysaccharides.