Genome sequence of an extremely halophilic archaeon isolated from Permian Period halite, Salado Formation in New Mexico, USA: Halobacterium sp. strain NMX12-1

Leonardo Soto; Priya DasSarma; Brian P Anton; Tamas Vincze; Ishita Verma; Bora Eralp; Dennis W Powers; Brian Lee Dozier; Richard J Roberts; Shiladitya DasSarma

doi:10.1128/mra.00778-24

Genome sequence of an extremely halophilic archaeon isolated from Permian Period halite, Salado Formation in New Mexico, USA: Halobacterium sp. strain NMX12-1

Microbiol Resour Announc. 2024 Nov 12;13(11):e0077824. doi: 10.1128/mra.00778-24. Epub 2024 Oct 21.

Authors

Leonardo Soto^{1

2

3}, Priya DasSarma^{2

4}, Brian P Anton⁵, Tamas Vincze⁵, Ishita Verma², Bora Eralp², Dennis W Powers⁶, Brian Lee Dozier⁷, Richard J Roberts⁵, Shiladitya DasSarma^{1

2

3

4}

Affiliations

¹ Marine Estuarine Environmental Sciences Program, University of Maryland, College Park, Maryland, USA.
² Blue Marble Space Institute of Science, Seattle, Washington, USA.
³ Institute of Marine and Environmental Technology, University System of Maryland, Baltimore, Maryland, USA.
⁴ Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, Maryland, USA.
⁵ New England Biolabs, Ipswich, Massachusetts, USA.
⁶ University of Mississippi, University, Mississippi, USA.
⁷ WIPP Test Coordination Office, Los Alamos National Laboratory (LANL), Carlsbad, New Mexico, USA.

Abstract

Halobacterium sp. strain NMX12-1, an extremely halophilic Archaeon, was isolated from 250 million-year-old Salado Formation salt crystal in Carlsbad, New Mexico. Single-molecule real-time sequencing revealed a 3.2-Mbp genome with a 2.6-Mbp chromosome and five plasmids (234, 211, 119, 21, and 1.6-kbp). The GC-rich genome encodes an acidic proteome, characteristic of Haloarchaea.

Keywords: DNA methylation; HOGs; Haloarchaea; acidic proteome; extremophile; halophile; insertion sequence; origin recognition complex; rhodopsin; transcription factors.