The Inducible TGFβ2 Ocular Hypertension Mouse Model

Methods Mol Biol. 2025:2858:123-129. doi: 10.1007/978-1-0716-4140-8_11.

Abstract

There is a great need for the development of mouse models of ocular hypertension. Here, we describe an inducible mouse model of ocular hypertension that produces a homogeneous intraocular pressure (IOP) response and can be induced in any mouse strain of choice without the need for extensive breeding. The procedure utilizes an ocular hypertension and glaucoma-relevant transgene, bioactivated TGFβ2, and an adenovirus 5 virus with select tropism for the trabecular meshwork (TM).

Keywords: IOP; Inducible model; Ocular hypertension; TGFβ2.

MeSH terms

  • Adenoviridae / genetics
  • Animals
  • Disease Models, Animal*
  • Genetic Vectors / administration & dosage
  • Genetic Vectors / genetics
  • Intraocular Pressure*
  • Mice
  • Mice, Transgenic
  • Ocular Hypertension* / metabolism
  • Trabecular Meshwork* / metabolism
  • Transforming Growth Factor beta2* / genetics
  • Transforming Growth Factor beta2* / metabolism
  • Transgenes

Substances

  • Transforming Growth Factor beta2