Innovative approaches in phenotypic beta-lactamase detection for personalised infection management

Nat Commun. 2024 Oct 21;15(1):9070. doi: 10.1038/s41467-024-53192-7.

Abstract

Beta-lactamase-producing Enterobacteriaceae present a significant therapeutic challenge. Current developments in phenotypic diagnostics focus primarily on rapid minimum inhibitory concentration (MIC) determination. There is a requirement for rapid phenotypic diagnostics to improve antimicrobial susceptibility tests (AST) and aid prescribing decisions. Phenotypic AST are limited in their ability to characterise beta-lactamase-producing Enterobacteriaceae in detail. Despite advances in rapid AST, gaps and opportunities remain for developing additional diagnostic approaches that facilitate personalised antimicrobial prescribing. In this perspective, we highlight the state-of-the-art in beta-lactamase detection, identify gaps in current practice, and discuss barriers for innovation within this field.

Publication types

  • Review

MeSH terms

  • Anti-Bacterial Agents* / pharmacology
  • Anti-Bacterial Agents* / therapeutic use
  • Enterobacteriaceae Infections* / diagnosis
  • Enterobacteriaceae Infections* / drug therapy
  • Enterobacteriaceae Infections* / microbiology
  • Enterobacteriaceae* / drug effects
  • Enterobacteriaceae* / enzymology
  • Enterobacteriaceae* / genetics
  • Humans
  • Microbial Sensitivity Tests*
  • Phenotype*
  • Precision Medicine / methods
  • beta-Lactamases* / genetics
  • beta-Lactamases* / metabolism

Substances

  • beta-Lactamases
  • Anti-Bacterial Agents