We investigated the dynamics of soil viral community in Cunninghamia lanceolata plantations with different stand ages (8, 21, 27, and 40 years old) in a subtropical region. The viral metagenomics and bioinformatics analysis were used to analyze the compositional and functional differences of soil viral communities across different stand ages, and to explore the environmental driving factors. The results showed that tailed phages dominated soil viral community in subtropical C. lanceolata plantations, with the highest proportion of Siphoviridae (19.6%-39.5%). There was significant difference in soil viral community structure among different stand ages, with the main driving factors being electrical conductance and available phosphorus. The metabolic functional genes encoded by viruses exhibited higher relative abundance. The α-diversity of soil viral function in mature C. lanceolata plantations was higher than other stands. There were significant differences in soil viral functional structure among different stand ages, which were mainly driven by ammonium nitrogen. During the development of C. lanceolata plantations, auxiliary metabolic genes encoded by virus related to nitrogen and phosphorus may regulate the metabolism of host microorganisms, thereby potentially impacting biogeochemical cycling of these elements.
为研究杉木人工林土壤病毒群落结构及功能随林分发育的动态演变特征,采集了亚热带不同林龄(8、21、27和40年生)的杉木人工林土壤样品,采用宏病毒组测序技术及生物信息学分析手段,分析土壤病毒群落结构及功能的变化,探讨二者的环境驱动因子。结果表明: 在亚热带杉木人工林,土壤病毒群落中有尾噬菌体的占比较高,其中长尾噬菌体科最多(19.6%～39.5%)。不同林龄杉木人工林土壤病毒群落结构差异显著,土壤病毒群落的主要驱动因子是电导率、有效磷。在亚热带杉木人工林土壤病毒编码的辅助代谢基因中,具有代谢功能的基因相对丰度最高,成熟杉木人工林土壤病毒功能的α多样性高于其他林分。不同林龄杉木人工林土壤病毒功能结构差异显著,土壤病毒功能的变化与NH4+-N显著相关。在杉木林林分发育过程中,病毒编码氮、磷相关的辅助代谢基因可能调控宿主微生物新陈代谢,从而对元素的生物地球化学循环过程产生潜在的影响。.
Keywords: Cunninghamia lanceolata plantation; auxiliary metabolic gene; soil virus; stand age.