A thiosulfate-oxidizing bacterium, Thiomicrospira sp. strain V2501, was isolated from groundwater collected in a terrestrial deep subsurface environment. This strain was capable of chemolithoautotrophic growth on CO2 and thiosulfate. Here, we report the 2,240,851 bp complete genome sequence of strain V2501.
Keywords: Thiomicrospira sp.; chemolithoautotrophy; subsurface environment.