Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly female for ageing research

Filipa Esteves; David Brito; Ana Teresa Rajado; Nádia Silva; Joana Apolónio; Vânia Palma Roberto; ALFA Score Consortium; Raquel P Andrade; Sofia Calado; Maria Leonor Faleiro; Carlos Matos; Nuno Marques; Ana Marreiros; Hipólito Nzwalo; Sandra Pais; Isabel Palmeirim; Sónia Simãoa; Natércia Joaquim; Rui Miranda; António Pêgas; Daniela Marques Raposo; Ana Sardo; Inês Araújo; Clévio Nóbrega; Pedro Castelo-Branco; José Bragança

doi:10.1016/j.scr.2024.103576

Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly female for ageing research

Stem Cell Res. 2024 Dec:81:103576. doi: 10.1016/j.scr.2024.103576. Epub 2024 Oct 15.

Authors

Filipa Esteves¹, David Brito¹, Ana Teresa Rajado¹, Nádia Silva¹, Joana Apolónio¹, Vânia Palma Roberto²; ALFA Score Consortium; Raquel P Andrade³, Sofia Calado⁴, Maria Leonor Faleiro⁵, Carlos Matos⁴, Nuno Marques⁴, Ana Marreiros⁴, Hipólito Nzwalo⁴, Sandra Pais⁴, Isabel Palmeirim³, Sónia Simãoa⁶, Natércia Joaquim⁷, Rui Miranda⁷, António Pêgas⁸, Daniela Marques Raposo⁹, Ana Sardo⁹, Inês Araújo¹⁰, Clévio Nóbrega¹⁰, Pedro Castelo-Branco¹⁰, José Bragança¹¹

Affiliations

¹ Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal.
² Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; ABC Collaborative Laboratory, Association for Integrated Ageing and Rejuvenation Solutions (ABC CoLAB), 8100-735 Loulé, Portugal.
³ Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Faculty of Medicine and Biomedical Sciences (FMCB), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Champalimaud Research Program, Champalimaud Centre for the Unknown, Avenida Brasília, 1400-038 Lisbon, Portugal.
⁴ Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Faculty of Medicine and Biomedical Sciences (FMCB), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal.
⁵ Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Faculty of Medicine and Biomedical Sciences (FMCB), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Faculty of Science and Technology (FCT), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal.
⁶ Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Faculty of Medicine and Biomedical Sciences (FMCB), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal.
⁷ USF Balsa, 8800-534 Tavira, Portugal.
⁸ USF Ossónoba, 8009-003 Faro, Portugal.
⁹ USF Mirante, 8700-240 Olhão, Portugal.
¹⁰ Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; ABC Collaborative Laboratory, Association for Integrated Ageing and Rejuvenation Solutions (ABC CoLAB), 8100-735 Loulé, Portugal; Faculty of Medicine and Biomedical Sciences (FMCB), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Champalimaud Research Program, Champalimaud Centre for the Unknown, Avenida Brasília, 1400-038 Lisbon, Portugal.
¹¹ Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; ABC Collaborative Laboratory, Association for Integrated Ageing and Rejuvenation Solutions (ABC CoLAB), 8100-735 Loulé, Portugal; Faculty of Medicine and Biomedical Sciences (FMCB), University of Algarve, Gambelas Campus, Bld. 2, 8005-139 Faro, Portugal; Champalimaud Research Program, Champalimaud Centre for the Unknown, Avenida Brasília, 1400-038 Lisbon, Portugal. Electronic address: [email protected].

PMID: 39437566
DOI: 10.1016/j.scr.2024.103576

Abstract

Human induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for development of an ageing score (ALFA Score). PBMCs were reprogrammed using three Sendai virus-based reprogramming vectors (hKOS, hc-Myc, and hKlf4). ABCRIi001-A showed normal morphology and karyotype, viral clearance, absence of genomic aberrations, and their pluripotency was confirmed by expression of pluripotency-related markers and their ability to differentiate into the three germ layers. ABCRIi001-A is valuable for ageing-related studies.

MeSH terms

Aged
Aging*
Cell Differentiation
Cell Line
Cellular Reprogramming
Female
Humans
Induced Pluripotent Stem Cells* / cytology
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear* / cytology
Leukocytes, Mononuclear* / metabolism
Sendai virus / genetics