Therapeutic Effects of Zanthoxyli Pericarpium on Intestinal Inflammation and Network Pharmacological Mechanism Analysis in a Dextran Sodium Sulfate-Induced Colitis Mouse Model

Nutrients. 2024 Oct 17;16(20):3521. doi: 10.3390/nu16203521.

Abstract

(1) Background: IBD (inflammatory bowel disease) is characterized by chronic intestinal inflammation leading to persistent symptoms and a lack of effective treatments. ZP (Zanthoxyli Pericarpium) has been used in traditional Chinese medicine for its anti-inflammatory and antioxidant properties for the management of intestinal disorders. (2) Methods: This study aimed to investigate the components of ZP, their specific targets, and associated diseases using the TCMSP (Traditional Chinese Medicine Systems Pharmacology) analysis platform, TCMBank database, and ETCM2.0 (Encyclopedia of Traditional Chinese Medicine 2.0) database. Additionally, we explored the protective effects of ZP on the colon and the underlying molecular mechanisms in the treatment of IBD. (3) Results: We identified 59 compounds in ZP that target 38 genes related to IBD, including PTGS2, PPARG, and GPBAR1. In a mice model of DSS (dextran sodium sulfate)-induced colitis, ZP significantly reduced colonic epithelial damage and oxidative stress markers, such as iNOS and nitrotyrosine, demonstrating its antioxidant properties. (4) Conclusions: These findings suggest that ZP has protective effects against DSS-induced colonic damage owing to its anti-inflammatory and antioxidant properties, making it a potential candidate for IBD treatment. However, further research and clinical trials are required to confirm its therapeutic potential and safety in humans.

Keywords: Zanthoxyli Pericarpium; colitis; dextran sodium sulfate; inflammatory bowel disease; network pharmacological; traditional Chinese medicine.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents* / pharmacology
  • Antioxidants* / pharmacology
  • Colitis* / chemically induced
  • Colitis* / drug therapy
  • Colon / drug effects
  • Colon / metabolism
  • Colon / pathology
  • Cyclooxygenase 2 / metabolism
  • Dextran Sulfate*
  • Disease Models, Animal*
  • Drugs, Chinese Herbal* / pharmacology
  • Inflammatory Bowel Diseases / chemically induced
  • Inflammatory Bowel Diseases / drug therapy
  • Male
  • Medicine, Chinese Traditional / methods
  • Mice
  • Mice, Inbred C57BL
  • Network Pharmacology
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / metabolism
  • Oxidative Stress / drug effects
  • PPAR gamma / metabolism
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism
  • Tyrosine / analogs & derivatives
  • Tyrosine / metabolism

Substances

  • Dextran Sulfate
  • Anti-Inflammatory Agents
  • Antioxidants
  • Drugs, Chinese Herbal
  • PPAR gamma
  • Pparg protein, mouse
  • Cyclooxygenase 2
  • Ptgs2 protein, mouse
  • Nitric Oxide Synthase Type II
  • Receptors, G-Protein-Coupled
  • Nos2 protein, mouse
  • Tyrosine
  • 3-nitrotyrosine