Monoclonal antibody development and antigenic epitope identification of infectious bursal disease virus VP5

Jinze Han; Xinxin Niu; Chengfei Ge; Ziwen Wu; Guodong Wang; Mengmeng Huang; Yulong Zhang; Runhang Liu; Mengmeng Xu; Hangbo Yu; Jingzhe Han; Suyan Wang; Yongzhen Liu; Yuntong Chen; Hongyu Cui; Yanping Zhang; Yulu Duan; Xiaomei Wang; Liuan Li; Yulong Gao; Xiaole Qi

doi:10.1016/j.tvjl.2024.106254

Monoclonal antibody development and antigenic epitope identification of infectious bursal disease virus VP5

Vet J. 2024 Oct 28:106254. doi: 10.1016/j.tvjl.2024.106254. Online ahead of print.

Authors

Jinze Han¹, Xinxin Niu², Chengfei Ge³, Ziwen Wu², Guodong Wang², Mengmeng Huang², Yulong Zhang², Runhang Liu², Mengmeng Xu¹, Hangbo Yu², Jingzhe Han¹, Suyan Wang⁴, Yongzhen Liu⁵, Yuntong Chen⁵, Hongyu Cui⁴, Yanping Zhang⁵, Yulu Duan⁵, Xiaomei Wang⁴, Liuan Li⁶, Yulong Gao⁷, Xiaole Qi⁸

Affiliations

¹ Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; World Organization for Animal Health (WOAH) Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.
² Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; World Organization for Animal Health (WOAH) Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, China.
³ Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, the Chinese Academy of Agricultural Sciences, Harbin 150069, China.
⁴ Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; World Organization for Animal Health (WOAH) Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China.
⁵ Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China.
⁶ Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China. Electronic address: [email protected].
⁷ Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; World Organization for Animal Health (WOAH) Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China. Electronic address: [email protected].
⁸ Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; World Organization for Animal Health (WOAH) Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China. Electronic address: [email protected].

PMID: 39477081
DOI: 10.1016/j.tvjl.2024.106254

Abstract

Infectious bursal disease (IBD) is an important immunosuppressive disease affecting chickens and is caused by infectious bursal disease virus (IBDV) infection. VP5 is a non-essential protein for IBDV replication but plays a critical role in IBDV pathogenesis. A deeper understanding of the biological functions of VP5 is lacking. This study utilized a prokaryotic system to express and purify soluble VP5 from the dominant epidemic strain of IBDV and developed a hybridoma cell line capable of secreting IBDV VP5 monoclonal antibody (MAb). The VP5 MAb demonstrated strong specificity for IBDV VP5 and could effectively discriminate between IBDV and its VP5-deleted strain. Furthermore, the antigen epitope of 137RRDLPKPE145 from IBDV VP5 was identified, which is the first detailed report of an IBDV VP5 antigen epitope. This antigen epitope, which is located at the C-terminus of VP5, is conserved across various IBDV serotype 1 strains. The findings of this study offer valuable insights for further exploration of gene function and differential detection of VP5.

Keywords: Antigen epitope; Infectious bursal disease virus; Monoclonal antibody; Soluble expression; VP5.