Coral-like AgNPs hybrided MOFs modulated with biopolymer polydopamine for synergistic antibacterial and biofilm eradication

Int J Biol Macromol. 2024 Dec;282(Pt 5):137080. doi: 10.1016/j.ijbiomac.2024.137080. Epub 2024 Oct 29.

Abstract

Bacterial contamination is an intractable challenge in food safety, environments and biomedicine fields, and places a heavy burden on society. Polydopamine (PDA), a high molecular biopolymer, is considered as a promising candidate to participate in the design of novel antibacterial agents with unique contributions in biocompatibility, adherence, photothermal and metal coordination ability. In this study, coral-like ZIFL-PDA@AgNPs with excellent antibacterial properties and biocompatibility were prepared by embedding AgNPs into the biopolymer PDA-modulated ZIFL-PDA nanostructures by green reduction method to solve the problem with poor stability of AgNPs. Based on the plasma resonance effect of AgNPs, coral-like ZIFL-PDA@AgNPs had enhanced photothermal properties compared with ZIFL-PDA. Due to the synergistic effect between antibacterial metal ions mainly Ag+ and the photothermal effect, coral-like ZIFL-PDA@AgNPs showed enhanced anti-mature biofilm and antibacterial properties, which was dependent on its concentration and sterilization time. In addition, regulated by the ZIFL-PDA nanostructure, coral-like ZIFL-PDA@AgNPs demonstrated a unique Ag+ long-time sustained release behavior, giving it an extended antibacterial validity period and good biocompatibility. Antibacterial mechanism experiments indicated that coral-like ZIFL-PDA@AgNPs can significantly damage the integrity of bacterial cell membrane, reduce the content of ATP in bacterial by affecting the activity of succinate dehydrogenase, and induce the accumulation of reactive oxygen species, ultimately leading to bacterial death.

Keywords: Antibacterial properties; Coral-like ZIFL-PDA@AgNPs; In situ reduction; Mechanism of antibacterial; Polydopamine; Synergistic effect.

MeSH terms

  • Animals
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Biofilms* / drug effects
  • Biopolymers / chemistry
  • Biopolymers / pharmacology
  • Escherichia coli / drug effects
  • Indoles* / chemistry
  • Indoles* / pharmacology
  • Metal Nanoparticles* / chemistry
  • Metal-Organic Frameworks* / chemistry
  • Metal-Organic Frameworks* / pharmacology
  • Microbial Sensitivity Tests
  • Polymers* / chemistry
  • Polymers* / pharmacology
  • Reactive Oxygen Species / metabolism
  • Silver* / chemistry
  • Silver* / pharmacology
  • Staphylococcus aureus / drug effects

Substances

  • Indoles
  • polydopamine
  • Anti-Bacterial Agents
  • Polymers
  • Silver
  • Metal-Organic Frameworks
  • Biopolymers
  • Reactive Oxygen Species