A prospective therapeutic strategy: GPX4-targeted ferroptosis mediators

Eur J Med Chem. 2025 Jan 5:281:117015. doi: 10.1016/j.ejmech.2024.117015. Epub 2024 Oct 29.

Abstract

As a crucial regulator of oxidative homeostasis, seleno-protein glutathione peroxidase 4 (GPX4) represents the primary defense system against ferroptosis, making it a promising target with important clinical application prospects. From the discovery of covalent and allosteric sites in GPX4, substantial advancements in GPX4-targeted small molecules have been made through diverse discovery and design strategies in recent years. Moreover, as an emerging hotspot in drug development, seleno-organic compounds can functionally mimic GPX4 to reduce hydroperoxides. To facilitate the further development of selective ferroptosis mediators as potential pharmaceutical agents, this review comprehensively covers all GPX4-targeted small molecules, including inhibitors, degraders, and activators. In addition, seleno-organic compounds as GPX mimics are also included. We also provide perspectives regarding challenges and future research directions in this field.

Keywords: Activator; Ferroptosis; GPX mimic; GPX4; Protein degrader.

Publication types

  • Review

MeSH terms

  • Animals
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology
  • Ferroptosis* / drug effects
  • Humans
  • Molecular Structure
  • Organoselenium Compounds / chemistry
  • Organoselenium Compounds / pharmacology
  • Phospholipid Hydroperoxide Glutathione Peroxidase* / antagonists & inhibitors
  • Phospholipid Hydroperoxide Glutathione Peroxidase* / metabolism

Substances

  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Enzyme Inhibitors
  • Organoselenium Compounds