A luminescent-based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood

STAR Protoc. 2024 Dec 20;5(4):103428. doi: 10.1016/j.xpro.2024.103428. Epub 2024 Nov 1.

Abstract

Here, we present a NAD+/NADH detection assay for evaluating NAD+, NADH, and NAD+/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from different models as well as detection and calculation of NAD+ and NADH levels. This protocol is applicable for quantifying cellular/tissue NAD+ and NADH levels across different biological models.

Keywords: Cell Biology; Metabolism; Molecular Biology; Neuroscience.

MeSH terms

  • Animals
  • Caenorhabditis elegans*
  • Humans
  • Luminescent Measurements / methods
  • Mice
  • NAD* / blood
  • NAD* / metabolism

Substances

  • NAD