The peroxidase gene OsPrx114 activated by OsWRKY50 enhances drought tolerance through ROS scavenging in rice

Chao Zheng; Xuming Wang; Yue Xu; Shaomin Wang; Xin Jiang; Xiuli Liu; Weijun Cui; Yueyan Wu; Chengqi Yan; Hongjia Liu; Yin Lu; Jianping Chen; Jie Zhou

doi:10.1016/j.plaphy.2023.108138

The peroxidase gene OsPrx114 activated by OsWRKY50 enhances drought tolerance through ROS scavenging in rice

Plant Physiol Biochem. 2023 Oct 24:204:108138. doi: 10.1016/j.plaphy.2023.108138. Online ahead of print.

Authors

Chao Zheng¹, Xuming Wang², Yue Xu³, Shaomin Wang⁴, Xin Jiang⁵, Xiuli Liu², Weijun Cui², Yueyan Wu⁶, Chengqi Yan³, Hongjia Liu², Yin Lu⁷, Jianping Chen⁸, Jie Zhou⁹

Affiliations

¹ College of Plant Protection, Northwest A&F University, Yangling, 712100, China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Ministry of Agriculture Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Ministry of Agriculture Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Ministry of Agriculture Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China; Zhejiang Wan Li University, Ningbo, 315100, China.
⁴ Agricultural Technology Extension and Service Station of Yuyao City, Yuyao, 315400, China.
⁵ College of Biological and Environment Engineering, Zhejiang Shuren University, Hangzhou, 310015, China.
⁶ Zhejiang Wan Li University, Ningbo, 315100, China.
⁷ College of Biological and Environment Engineering, Zhejiang Shuren University, Hangzhou, 310015, China. Electronic address: [email protected].
⁸ College of Plant Protection, Northwest A&F University, Yangling, 712100, China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Ministry of Agriculture Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. Electronic address: [email protected].
⁹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Ministry of Agriculture Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. Electronic address: [email protected].

PMID: 39492168
DOI: 10.1016/j.plaphy.2023.108138

Abstract

Drought is among the most severe environmental stressors, imposing detrimental effects on plant growth and development. In this study, we have identified a class III peroxidase gene, OsPrx114, which was induced under PEG (Polyethylene glycol) and drought conditions and found to be localized in both the plasma membrane and endoplasmic reticulum. The promoter region of OsPrx114 encompasses cis-elements, including ABRE (ABA response elements), MBS (MYB binding elements), and W-box, which are likely contributors to drought tolerance. In comparison to Nipponbare (Nip) plants, the overexpression of OsPrx114 enhanced drought tolerance by reducing the accumulation of reactive oxygen species (ROS) through the upregulation of enzyme activities, such as peroxide dismutase (POD) and catalase (CAT). This was accompanied by an increase in proline (Pro) content and a decrease in malondialdehyde (MDA) content. Furthermore, a series of assays including yeast one-hybrid, electrophoretic mobility shift, and dual luciferase assays, demonstrated that the transcription factor OsWRKY50 binds to the W-box (TTGACC) within the promoter of OsPrx114, thereby activating its transcription. OsWRKY50 plays a positive role in regulating OsPrx114-mediated drought resistance by mitigating ROS accumulation in rice. These findings offer a molecular foundation for comprehending the function of the OsWRKY50-OsPrx114 module in response to drought stress in rice.

Keywords: Drought tolerance; OsPrx114; OsWRKY50; ROS scavenging; Rice.