RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection

Takumi Tezuka; Misuzu Nosaka-Takahashi; Takanori Yoshikawa; Yutaka Sato

doi:10.1007/978-1-0716-4204-7_4

RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection

Methods Mol Biol. 2025:2869:21-27. doi: 10.1007/978-1-0716-4204-7_4.

Authors

Takumi Tezuka¹, Misuzu Nosaka-Takahashi², Takanori Yoshikawa², Yutaka Sato²

Affiliations

¹ National Institute of Genetics, Mishima, Shizuoka, Japan. [email protected].
² National Institute of Genetics, Mishima, Shizuoka, Japan.

PMID: 39499464
DOI: 10.1007/978-1-0716-4204-7_4

Abstract

Laser capture microdissection (LCM) enables the selective isolation of organs, tissues, and cells from surrounding tissues. Total RNA extracted from small tissue sections can be used for a variety of subsequent analysis such as RNA-seq analysis. Here, we describe a method for isolating embryos from rice ovary sections using LCM and extracting total RNA. We successfully obtained sufficient amount of total RNA from a single rice embryo at the globular embryo stage for RNA-seq analysis.

Keywords: Embryo; Laser capture microdissection (LCM); Paraffin section; RNA extraction; RNA-seq.

MeSH terms

Laser Capture Microdissection* / methods
Oryza* / embryology
Oryza* / genetics
RNA, Plant* / genetics
RNA, Plant* / isolation & purification
Seeds* / genetics

Substances

RNA, Plant