Characterisation of a Mesophilic Aeromonas salmonicida and the Development of a PCR to Differentiate Atypical and Typical Strains

J Fish Dis. 2025 Jan;48(1):e14028. doi: 10.1111/jfd.14028. Epub 2024 Nov 6.

Abstract

This study describes the identification and characterisation of a new mesophilic Aeromonas salmonicida strain, named HMes1 isolated from Atlantic salmon (Salmo salar L.) in Tasmania. Isolates were identified as Aeromonas salmonicida through phenotypic, phylogenetic and genetic characterisation. After characterisation, the diagnostic phenotypic identification system MicroSys A24 was updated and a new multiplex conventional PCR was developed to enable rapid and inexpensive identification of atypical A. salmonicida, and exclusion of the exotic strain, A. salmonicida ssp. salmonicida.

Keywords: Aeromonas; PCR; characterisation; salmonicida.

MeSH terms

  • Aeromonas salmonicida* / classification
  • Aeromonas salmonicida* / genetics
  • Aeromonas salmonicida* / isolation & purification
  • Animals
  • Fish Diseases* / diagnosis
  • Fish Diseases* / microbiology
  • Gram-Negative Bacterial Infections* / diagnosis
  • Gram-Negative Bacterial Infections* / microbiology
  • Gram-Negative Bacterial Infections* / veterinary
  • Phylogeny*
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary
  • RNA, Ribosomal, 16S / genetics
  • Salmo salar* / microbiology
  • Tasmania

Substances

  • RNA, Ribosomal, 16S