A self-enhanced electrochemiluminescence aptasensor Zr-porphyrin modified with polyamidoamine for sensitive detection of lincomycin

Food Chem. 2025 Feb 1;464(Pt 3):141846. doi: 10.1016/j.foodchem.2024.141846. Epub 2024 Oct 29.

Abstract

Exploring novel and sensitive analysis methods for monitoring lincomycin (Lin) residues is of great significance since overuse of it would cause a serious threat to public health. Herein, a Zr-porphyrin metal-organic frameworks (Zr-TCPP) with covalently modified polyamidoamine (PAMAM) dendrimers was synthesized as a novel intramolecular self-enhanced ECL reagent, which exhibited greatly improved ECL response due to the promotion of SO4•- generation and the shortening of the electron transfer distance. Graphene oxide modified with gold nanoparticles (Au@GO) was synthesized as the quencher for the ECL sensor construction based on the quenching strategy. The present aptasensor achieved a wide linear range of 1.0 × 10-14 - 5.0 × 10-9 g/mL and a low detection limit of 1.7 fg/mL, which was applied for the determination of Lin in different real samples with satisfactory results.

Keywords: Electrochemilunescence sensor; Lincomycin; Self-enhancement; Zr-TCPP.

Publication types

  • Evaluation Study

MeSH terms

  • Aptamers, Nucleotide / chemistry
  • Biosensing Techniques / instrumentation
  • Electrochemical Techniques*
  • Food Contamination / analysis
  • Gold / chemistry
  • Graphite / chemistry
  • Limit of Detection
  • Lincomycin* / analysis
  • Lincomycin* / chemistry
  • Luminescent Measurements / instrumentation
  • Luminescent Measurements / methods
  • Metal Nanoparticles / chemistry
  • Metal-Organic Frameworks / chemistry
  • Polyamines / chemistry
  • Porphyrins* / chemistry
  • Zirconium* / chemistry

Substances

  • Lincomycin
  • Porphyrins
  • Zirconium
  • Graphite
  • Gold
  • Metal-Organic Frameworks
  • Poly(amidoamine)
  • Aptamers, Nucleotide
  • Polyamines